Baljuls A, et al. (2008) Positive regulation of A-RAF by phosphorylation of isoform-specific hinge segment and identification of novel phosphorylation sites. J Biol Chem 283, 27239-54 18662992

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

S15-p - ARAF (human) ▲
Modsite: PANGAEPsRAVGtVK SwissProt Entrez-Gene Orthologous residues ARAF (human): S15‑p, ARAF iso2 (human): S15‑p, ARAF (mouse): S15‑p, ARAF (rat): S15‑p, ARAF (cow): S15‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

T20-p - ARAF (human) ▲
Modsite: EPsRAVGtVKVyLPN SwissProt Entrez-Gene Orthologous residues ARAF (human): T20‑p, ARAF iso2 (human): T20‑p, ARAF (mouse): T20‑p, ARAF (rat): T20‑p, ARAF (cow): T20‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

Y24-p - ARAF (human) ▲
Modsite: AVGtVKVyLPNkQRT SwissProt Entrez-Gene Orthologous residues ARAF (human): Y24‑p, ARAF iso2 (human): Y24‑p, ARAF (mouse): Y24‑p, ARAF (rat): Y24‑p, ARAF (cow): Y24‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S214-p - ARAF (human) ▲

Modsite: LQRIRststPNVHMV SwissProt Entrez-Gene Orthologous residues ARAF (human): S214‑p, ARAF iso2 (human): ‑, ARAF (mouse): S214‑p, ARAF (rat): S214‑p, ARAF (cow): S214‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody Relevant cell lines - cell types - tissues:  SF9 [Lck (human)] Cellular systems studied:  cell lines Species studied:  insect Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S250-p - ARAF (human) ▲
Modsite: AAGSRGGsDGtPRGs SwissProt Entrez-Gene Orthologous residues ARAF (human): S250‑p, ARAF iso2 (human): ‑, ARAF (mouse): ‑, ARAF (rat): ‑, ARAF (cow): G250‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

T253-p - ARAF (human) ▲
Modsite: SRGGsDGtPRGsPsP SwissProt Entrez-Gene Orthologous residues ARAF (human): T253‑p, ARAF iso2 (human): ‑, ARAF (mouse): A251‑p, ARAF (rat): A251‑p, ARAF (cow): A253‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S257-p - ARAF (human) ▲

Modsite: sDGtPRGsPsPAsVs SwissProt Entrez-Gene Orthologous residues ARAF (human): S257‑p, ARAF iso2 (human): ‑, ARAF (mouse): S255‑p, ARAF (rat): S255‑p, ARAF (cow): S257‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S259-p - ARAF (human) ▲
Modsite: GtPRGsPsPAsVssG SwissProt Entrez-Gene Orthologous residues ARAF (human): S259‑p, ARAF iso2 (human): ‑, ARAF (mouse): S257‑p, ARAF (rat): S257‑p, ARAF (cow): S259‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S262-p - ARAF (human) ▲

Modsite: RGsPsPAsVssGRKs SwissProt Entrez-Gene Orthologous residues ARAF (human): S262‑p, ARAF iso2 (human): ‑, ARAF (mouse): S260‑p, ARAF (rat): S260‑p, ARAF (cow): S262‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S264-p - ARAF (human) ▲

Modsite: sPsPAsVssGRKsPH SwissProt Entrez-Gene Orthologous residues ARAF (human): S264‑p, ARAF iso2 (human): ‑, ARAF (mouse): S262‑p, ARAF (rat): S262‑p, ARAF (cow): S264‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S265-p - ARAF (human) ▲
Modsite: PsPAsVssGRKsPHs SwissProt Entrez-Gene Orthologous residues ARAF (human): S265‑p, ARAF iso2 (human): ‑, ARAF (mouse): S263‑p, ARAF (rat): S263‑p, ARAF (cow): S265‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S269-p - ARAF (human) ▲
Modsite: sVssGRKsPHsKsPA SwissProt Entrez-Gene Orthologous residues ARAF (human): S269‑p, ARAF iso2 (human): ‑, ARAF (mouse): S267‑p, ARAF (rat): S267‑p, ARAF (cow): S269‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S272-p - ARAF (human) ▲
Modsite: sGRKsPHsKsPAEQR SwissProt Entrez-Gene Orthologous residues ARAF (human): S272‑p, ARAF iso2 (human): ‑, ARAF (mouse): S270‑p, ARAF (rat): S270‑p, ARAF (cow): S272‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S274-p - ARAF (human) ▲
Modsite: RKsPHsKsPAEQRER SwissProt Entrez-Gene Orthologous residues ARAF (human): S274‑p, ARAF iso2 (human): ‑, ARAF (mouse): L272‑p, ARAF (rat): L272‑p, ARAF (cow): S274‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S283-p - ARAF (human) ▲
Modsite: AEQRERksLADDKKK SwissProt Entrez-Gene Orthologous residues ARAF (human): S283‑p, ARAF iso2 (human): ‑, ARAF (mouse): S281‑p, ARAF (rat): S281‑p, ARAF (cow): S283‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

T318-p - ARAF (human) ▲
Modsite: QLLkRIGtGsFGtVF SwissProt Entrez-Gene Orthologous residues ARAF (human): T318‑p, ARAF iso2 (human): ‑, ARAF (mouse): T316‑p, ARAF (rat): T316‑p, ARAF (cow): T318‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S320-p - ARAF (human) ▲
Modsite: LkRIGtGsFGtVFRG SwissProt Entrez-Gene Orthologous residues ARAF (human): S320‑p, ARAF iso2 (human): ‑, ARAF (mouse): S318‑p, ARAF (rat): S318‑p, ARAF (cow): S320‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

T323-p - ARAF (human) ▲
Modsite: IGtGsFGtVFRGRWH SwissProt Entrez-Gene Orthologous residues ARAF (human): T323‑p, ARAF iso2 (human): ‑, ARAF (mouse): T321‑p, ARAF (rat): T321‑p, ARAF (cow): T323‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S341-p - ARAF (human) ▲
Modsite: AVKVLkVsQPtAEQA SwissProt Entrez-Gene Orthologous residues ARAF (human): S341‑p, ARAF iso2 (human): ‑, ARAF (mouse): A339‑p, ARAF (rat): A339‑p, ARAF (cow): A341‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

T344-p - ARAF (human) ▲
Modsite: VLkVsQPtAEQAQAF SwissProt Entrez-Gene Orthologous residues ARAF (human): T344‑p, ARAF iso2 (human): ‑, ARAF (mouse): T342‑p, ARAF (rat): T342‑p, ARAF (cow): T344‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

T413-p - ARAF (human) ▲
Modsite: LIDVARQtAQGMDyL SwissProt Entrez-Gene Orthologous residues ARAF (human): T413‑p, ARAF iso2 (human): ‑, ARAF (mouse): T411‑p, ARAF (rat): T411‑p, ARAF (cow): T413‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

Y419-p - ARAF (human) ▲
Modsite: QtAQGMDyLHAkNII SwissProt Entrez-Gene Orthologous residues ARAF (human): Y419‑p, ARAF iso2 (human): ‑, ARAF (mouse): Y417‑p, ARAF (rat): Y417‑p, ARAF (cow): Y419‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S432-p - ARAF (human) ▲

Modsite: IIHRDLksNNIFLHE SwissProt Entrez-Gene Orthologous residues ARAF (human): S432‑p, ARAF iso2 (human): ‑, ARAF (mouse): S430‑p, ARAF (rat): S430‑p, ARAF (cow): S432‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced

T442-p - ARAF (human) ▲

Modsite: IFLHEGLtVKIGDFG SwissProt Entrez-Gene Orthologous residues ARAF (human): T442‑p, ARAF iso2 (human): ‑, ARAF (mouse): T440‑p, ARAF (rat): T440‑p, ARAF (cow): T442‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey Downstream Regulation Effect of modification (function):  enzymatic activity, induced

T452-p - ARAF (human) ▲

Modsite: IGDFGLAtVktRWsG SwissProt Entrez-Gene Orthologous residues ARAF (human): T452‑p, ARAF iso2 (human): ‑, ARAF (mouse): T450‑p, ARAF (rat): T450‑p, ARAF (cow): T452‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey Downstream Regulation Effect of modification (function):  enzymatic activity, induced

T455-p - ARAF (human) ▲

Modsite: FGLAtVktRWsGAQP SwissProt Entrez-Gene Orthologous residues ARAF (human): T455‑p, ARAF iso2 (human): ‑, ARAF (mouse): T453‑p, ARAF (rat): T453‑p, ARAF (cow): T455‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey Downstream Regulation Effect of modification (function):  enzymatic activity, induced

Y526-p - ARAF (human) ▲
Modsite: IFMVGRGyLsPDLsK SwissProt Entrez-Gene Orthologous residues ARAF (human): Y526‑p, ARAF iso2 (human): ‑, ARAF (mouse): Y524‑p, ARAF (rat): Y524‑p, ARAF (cow): Y526‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S528-p - ARAF (human) ▲
Modsite: MVGRGyLsPDLsKIs SwissProt Entrez-Gene Orthologous residues ARAF (human): S528‑p, ARAF iso2 (human): ‑, ARAF (mouse): S526‑p, ARAF (rat): S526‑p, ARAF (cow): S528‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S532-p - ARAF (human) ▲
Modsite: GyLsPDLsKIssNCP SwissProt Entrez-Gene Orthologous residues ARAF (human): S532‑p, ARAF iso2 (human): ‑, ARAF (mouse): S530‑p, ARAF (rat): S530‑p, ARAF (cow): S532‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S535-p - ARAF (human) ▲
Modsite: sPDLsKIssNCPkAM SwissProt Entrez-Gene Orthologous residues ARAF (human): S535‑p, ARAF iso2 (human): ‑, ARAF (mouse): F533‑p, ARAF (rat): F533‑p, ARAF (cow): S535‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S536-p - ARAF (human) ▲
Modsite: PDLsKIssNCPkAMR SwissProt Entrez-Gene Orthologous residues ARAF (human): S536‑p, ARAF iso2 (human): ‑, ARAF (mouse): S534‑p, ARAF (rat): S534‑p, ARAF (cow): S536‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S547-p - ARAF (human) ▲
Modsite: kAMRRLLsDCLkFQR SwissProt Entrez-Gene Orthologous residues ARAF (human): S547‑p, ARAF iso2 (human): ‑, ARAF (mouse): T545‑p, ARAF (rat): T545‑p, ARAF (cow): S547‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S573-p - ARAF (human) ▲
Modsite: TIELLQRsLPkIERs SwissProt Entrez-Gene Orthologous residues ARAF (human): S573‑p, ARAF iso2 (human): ‑, ARAF (mouse): S571‑p, ARAF (rat): S571‑p, ARAF (cow): S573‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S580-p - ARAF (human) ▲
Modsite: sLPkIERsAsEPsLH SwissProt Entrez-Gene Orthologous residues ARAF (human): S580‑p, ARAF iso2 (human): ‑, ARAF (mouse): S578‑p, ARAF (rat): S578‑p, ARAF (cow): S580‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S582-p - ARAF (human) ▲

Modsite: PkIERsAsEPsLHRt SwissProt Entrez-Gene Orthologous residues ARAF (human): S582‑p, ARAF iso2 (human): ‑, ARAF (mouse): S580‑p, ARAF (rat): S580‑p, ARAF (cow): S582‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, phospho-antibody Relevant cell lines - cell types - tissues:  COS (fibroblast), SF9 [Lck (human)] Cellular systems studied:  cell lines Species studied:  green monkey, insect Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S585-p - ARAF (human) ▲
Modsite: ERsAsEPsLHRtQAD SwissProt Entrez-Gene Orthologous residues ARAF (human): S585‑p, ARAF iso2 (human): ‑, ARAF (mouse): S583‑p, ARAF (rat): S583‑p, ARAF (cow): S585‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

T589-p - ARAF (human) ▲
Modsite: sEPsLHRtQADELPA SwissProt Entrez-Gene Orthologous residues ARAF (human): T589‑p, ARAF iso2 (human): ‑, ARAF (mouse): T587‑p, ARAF (rat): T587‑p, ARAF (cow): T589‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey

S600-p - ARAF (human) ▲
Modsite: ELPACLLsAARLVP_ SwissProt Entrez-Gene Orthologous residues ARAF (human): S600‑p, ARAF iso2 (human): ‑, ARAF (mouse): S598‑p, ARAF (rat): S598‑p, ARAF (cow): S600‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey