Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 18614797
Sarker R, et al. (2008) Casein kinase 2 binds to the C terminus of Na+/H+ exchanger 3 (NHE3) and stimulates NHE3 basal activity by phosphorylating a separate site in NHE3. Mol Biol Cell 19, 3859-70 18614797
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S554-p - NHE3 (rabbit)
Modsite: TEGERRGsLAFIRSP SwissProt Entrez-Gene
Orthologous residues
NHE3 (human): S555‑p, NHE3 (mouse): S550‑p, NHE3 (rat): S552‑p, NHE3 (rabbit): S554‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme

S719-p - NHE3 (rabbit)
Modsite: KERELELsDPEEAPD SwissProt Entrez-Gene
Orthologous residues
NHE3 (human): S718‑p, NHE3 (mouse): S714‑p, NHE3 (rat): S716‑p, NHE3 (rabbit): S719‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  Caco-2 (intestinal), PS120 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TBB decrease
DMAT decrease
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization