Curated Information
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Home > Curated Information Page > PubMed Id: 9312068
Lu X, Nemoto S, Lin A (1997) Identification of c-Jun NH2-terminal protein kinase (JNK)-activating kinase 2 as an activator of JNK but not p38. J Biol Chem 272, 24751-4 9312068
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T183-p - JNK1 (human)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MKK7 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK7 (human) transfection of wild-type enzyme, activation of upstream enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  transcription, altered

Y185-p - JNK1 (human)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MKK7 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK7 (human) transfection of wild-type enzyme, activation of upstream enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  transcription, altered

S271-p - MKK7 (human)
Modsite: IsGRLVDsKAKtRsA SwissProt Entrez-Gene
Orthologous residues
MKK7 (human): S271‑p, MKK7 (mouse): S287‑p, MKK7 iso2 (mouse): S271‑p, MKK7 iso6 (mouse): S287‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK1 (human) transfection of wild-type enzyme, activation of upstream enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  transcription, altered

T275-p - MKK7 (human)
Modsite: LVDsKAKtRsAGCAA SwissProt Entrez-Gene
Orthologous residues
MKK7 (human): T275‑p, MKK7 (mouse): T291‑p, MKK7 iso2 (mouse): T275‑p, MKK7 iso6 (mouse): T291‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK1 (human) transfection of wild-type enzyme, activation of upstream enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  transcription, altered