Curated Information
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Home > Curated Information Page > PubMed Id: 16682956
Mitra SK, et al. (2006) Intrinsic FAK activity and Y925 phosphorylation facilitate an angiogenic switch in tumors. Oncogene 25, 5969-84 16682956
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  4T1 (breast cell), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FAK iso9 (mouse) decrease FRNK expression

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  4T1 (breast cell), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FAK iso9 (mouse) decrease FRNK expression

Y397-p - FAK (mouse)
Modsite: sVsEtDDyAEIIDEE SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y397‑p, FAK iso2 (mouse): Y428‑p, FAK iso4 (mouse): Y397‑p, FAK iso9 (mouse): , FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  4T1 (breast cell), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FAK iso9 (mouse) no change compared to control FRNK expression

Y576-p - FAK (mouse)
Modsite: RyMEDstyyKASKGK SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y576‑p, FAK iso2 (human): Y424‑p, FAK iso5 (human): Y576‑p, FAK (mouse): Y576‑p, FAK iso2 (mouse): Y607‑p, FAK iso4 (mouse): Y576‑p, FAK iso9 (mouse): , FAK (rat): Y576‑p, FAK (chicken): Y576‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  4T1 (breast cell), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FAK iso9 (mouse) decrease FRNK expression

Y577-p - FAK (mouse)
Modsite: yMEDstyyKASKGKL SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y577‑p, FAK iso2 (human): Y425‑p, FAK iso5 (human): Y577‑p, FAK (mouse): Y577‑p, FAK iso2 (mouse): Y608‑p, FAK iso4 (mouse): Y577‑p, FAK iso9 (mouse): , FAK (rat): Y577‑p, FAK (chicken): Y577‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  4T1 (breast cell), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FAK iso9 (mouse) decrease FRNK expression

Y861-p - FAK (mouse)
Modsite: PTGNQHIyQPVGKPD SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y861‑p, FAK iso2 (human): Y688‑p, FAK iso5 (human): Y861‑p, FAK (mouse): Y861‑p, FAK iso2 (mouse): Y892‑p, FAK iso4 (mouse): Y861‑p, FAK iso9 (mouse): Y169‑p, FAK (rat): Y861‑p, FAK (chicken): Y863‑p, FAK iso5 (chicken): Y169‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  4T1 (breast cell), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FAK iso9 (mouse) decrease FRNK expression

Y925-p - FAK (mouse)
Modsite: DRSNDKVyENVTGLV SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y925‑p, FAK iso2 (human): Y752‑p, FAK iso5 (human): Y938‑p, FAK (mouse): Y925‑p, FAK iso2 (mouse): Y956‑p, FAK iso4 (mouse): Y928‑p, FAK iso9 (mouse): Y233‑p, FAK (rat): Y928‑p, FAK (chicken): Y926‑p, FAK iso5 (chicken): Y232‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  4T1 (breast cell), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FAK iso9 (mouse) decrease FRNK expression
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  cell growth, altered
Comments:  ERK phosphorylation/activation; increases tumor size

S218-p - MEK1 (mouse)
Modsite: VSGQLIDsMANsFVG SwissProt Entrez-Gene
Orthologous residues
MEK1 (human): S218‑p, MEK1 iso2 (human): S192‑p, MEK1 (mouse): S218‑p, MEK1 (rat): S218‑p, MEK1 (rabbit): S218‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  4T1 (breast cell), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FAK iso9 (mouse) decrease FRNK expression

S222-p - MEK1 (mouse)
Modsite: LIDsMANsFVGTRSY SwissProt Entrez-Gene
Orthologous residues
MEK1 (human): S222‑p, MEK1 iso2 (human): S196‑p, MEK1 (mouse): S222‑p, MEK1 (rat): S222‑p, MEK1 (rabbit): S222‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  4T1 (breast cell), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FAK iso9 (mouse) decrease FRNK expression

S298-p - MEK1 (mouse)
Modsite: RtPGRPLsSYGMDSR SwissProt Entrez-Gene
Orthologous residues
MEK1 (human): S298‑p, MEK1 iso2 (human): S272‑p, MEK1 (mouse): S298‑p, MEK1 (rat): S298‑p, MEK1 (rabbit): S298‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  4T1 (breast cell), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FAK iso9 (mouse) no change compared to control FRNK expression