Curated Information
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Home > Curated Information Page > PubMed Id: 24629717
Gl├╝ck L, et al. (2014) Loss of morphine reward and dependence in mice lacking G protein-coupled receptor kinase 5. Biol Psychiatry 76, 767-74 24629717
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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T370-p - MOR-1 (mouse)
Modsite: sARIRQNtREHPstA SwissProt Entrez-Gene
Orthologous residues
MOR‑1 (human): T372‑p, MOR‑1 iso5 (human): T372‑p, MOR‑1 (mouse): T370‑p, MOR‑1 (rat): T370‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
morphine no change compared to control
etonitazene increase
fentanyl increase
fentanyl, mutation no change compared to control S375A

S375-p - MOR-1 (mouse)
Modsite: QNtREHPstANtVDR SwissProt Entrez-Gene
Orthologous residues
MOR‑1 (human): S377‑p, MOR‑1 iso5 (human): S377‑p, MOR‑1 (mouse): S375‑p, MOR‑1 (rat): S375‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GRK3 (mouse) genetic knockout/knockin of upstream enzyme
KINASE GRK5 (mouse) genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
morphine increase
etonitazene increase
fentanyl increase
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  signaling pathway regulation
Comments:  influence opioid-related behaviors

T379-p - MOR-1 (mouse)
Modsite: EHPstANtVDRTNHQ SwissProt Entrez-Gene
Orthologous residues
MOR‑1 (human): T381‑p, MOR‑1 iso5 (human): T381‑p, MOR‑1 (mouse): T379‑p, MOR‑1 (rat): T379‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
morphine no change compared to control
etonitazene increase
fentanyl increase
fentanyl, mutation no change compared to control S375A