Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 24632950
Park MT, et al. (2014) Novel signaling axis for ROS generation during K-Ras-induced cellular transformation. Cell Death Differ 21, 1185-97 24632950
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S345-p - p47phox (human)
Modsite: QARPGPQsPGsPLEE SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S345‑p, p47phox (mouse): S346‑p, p47phox (rat): S346‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat

S348-p - p47phox (human)
Modsite: PGPQsPGsPLEEERQ SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S348‑p, p47phox (mouse): G349‑p, p47phox (rat): S349‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCD (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCD (human) co-immunoprecipitation, transfection of dominant-negative enzyme, phospho-antibody
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization
Effect of modification (process):  carcinogenesis, induced, cell growth, induced
Comments:  induces NOX1 activation and ROS generation

S359-p - p47phox (human)
Modsite: EERQtQRsKPQPAVP SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S359‑p, p47phox (mouse): V359‑p, p47phox (rat): A358‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat

S370-p - p47phox (human)
Modsite: PAVPPRPsADLILNR SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S370‑p, p47phox (mouse): S370‑p, p47phox (rat): S369‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat

S379-p - p47phox (human)
Modsite: DLILNRCsEstKRKL SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S379‑p, p47phox (mouse): T379‑p, p47phox (rat): T378‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCD (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCD (human) co-immunoprecipitation, transfection of dominant-negative enzyme, phospho-antibody
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization
Effect of modification (process):  carcinogenesis, induced, cell growth, induced
Comments:  induces NOX1 activation and ROS generation

Y313-p - PKCD (human)
Modsite: ssEPVGIyQGFEKkT SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y313‑p, PKCD iso2 (human): Y313‑p, PKCD (mouse): Y311‑p, PKCD iso2 (mouse): Y311‑p, PKCD (rat): Y311‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat

T507-p - PKCD (human)
Modsite: FGEsRAstFCGtPDy SwissProt Entrez-Gene
Orthologous residues
PKCD (human): T507‑p, PKCD iso2 (human): T538‑p, PKCD (mouse): T505‑p, PKCD iso2 (mouse): T531‑p, PKCD (rat): T505‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PDK1 (human) co-immunoprecipitation, siRNA inhibition of enzyme, phospho-antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KRas (human) increase
P38A (human) KRas (human) inhibit treatment-induced increase dominant negative p38 mutant
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

Y525-p - PKCD (human)
Modsite: EILQGLKyTFSVDWW SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y525‑p, PKCD iso2 (human): Y556‑p, PKCD (mouse): Y523‑p, PKCD iso2 (mouse): Y549‑p, PKCD (rat): Y523‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat

Y567-p - PKCD (human)
Modsite: IRVDTPHyPRWITKE SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y567‑p, PKCD iso2 (human): Y598‑p, PKCD (mouse): Y565‑p, PKCD iso2 (mouse): Y591‑p, PKCD (rat): Y565‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat