Curated Information
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Home > Curated Information Page > PubMed Id: 24586906
Yamada E, Bastie CC (2014) Disruption of Fyn SH3 Domain Interaction with a Proline-Rich Motif in Liver Kinase B1 Results in Activation of AMP-Activated Protein Kinase. PLoS One 9, e89604 24586906
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S79-p - ACC1 (mouse)
Modsite: FHMRSsMsGLHLVKQ SwissProt Entrez-Gene
Orthologous residues
ACC1 (human): S80‑p, ACC1 iso2 (human): S22‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', C2C12 (myoblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (human) phospho-antibody, activation of upstream enzyme

T172-p - AMPKA2 (mouse)
Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p, AMPKA2 (pig): T172‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', C2C12 (myoblast)
Cellular systems studied:  cell lines, tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
acadesine increase

Y261-p - LKB1 (mouse)
Modsite: PFEGDNIyKLFENIG SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): Y261‑p, LKB1 iso2 (human): Y261‑p, LKB1 (mouse): Y261‑p, LKB1 (rat): Y261‑p, LKB1 (pig): Y167‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Fyn (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Fyn (human) SH3 Induces intracellular localization pull-down assay, in vitro
Comments:  LKB1 cytosolic localization, induced AMPK phosphorylation

Y365-p - LKB1 (mouse)
Modsite: DIEDGIIytQDFTVP SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): Y362‑p, LKB1 iso2 (human): Y362‑p, LKB1 (mouse): Y365‑p, LKB1 (rat): Y365‑p, LKB1 (pig): Y270‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Fyn (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Fyn (human) SH3 Induces intracellular localization pull-down assay, in vitro
Comments:  LKB1 cytosolic localization, induced AMPK phosphorylation