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Home > Curated Information Page > PubMed Id: 23542178
Ducker GS, et al. (2014) Incomplete inhibition of phosphorylation of 4E-BP1 as a mechanism of primary resistance to ATP-competitive mTOR inhibitors. Oncogene 33, 1590-600 23542178
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T37-p - 4E-BP1 (human)
Modsite: PPGDysttPGGtLFs SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p, 4E‑BP1 (cow): T37‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP242 no change compared to control in SW620 cell line
PD0325901 PP242 no change compared to control
FMK-MEA PP242 no change compared to control
PP242 decrease in HCT15 and SW48 cell lines
PD0325901 PP242 no effect upon treatment-induced decrease
FMK-MEA PP242 no effect upon treatment-induced decrease
rapamycin no change compared to control in HCT15 cell line

T46-p - 4E-BP1 (human)
Modsite: GGtLFsttPGGtRII SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p, 4E‑BP1 (cow): T46‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP242 no change compared to control in SW620 cell line
PD0325901 PP242 no change compared to control
FMK-MEA PP242 no change compared to control
PP242 decrease in HCT15 and SW48 cell lines
PD0325901 PP242 no effect upon treatment-induced decrease
FMK-MEA PP242 no effect upon treatment-induced decrease
rapamycin no change compared to control in HCT15 cell line

S65-p - 4E-BP1 (human)
Modsite: FLMECrNsPVtktPP SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): S65‑p, 4E‑BP1 (mouse): S64‑p, 4E‑BP1 (rat): S64‑p, 4E‑BP1 (fruit fly): S65‑p, 4E‑BP1 (cow): S65‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP242 decrease in HCT15 cell line
PP242 no change compared to control in SW620 cell line

T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP242 decrease in SW620 HCT15 and SW48 cell lines
PD0325901 PP242 no effect upon treatment-induced decrease
FMK-MEA PP242 no effect upon treatment-induced decrease
rapamycin no change compared to control in HCT15 cell line

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP242 decrease in SW620 HCT15 and SW48 cell lines
PD0325901 PP242 no effect upon treatment-induced decrease
FMK-MEA PP242 no effect upon treatment-induced decrease
KU-0063794 decrease in SW620 and HCT15 cell lines
INK-128 decrease in SW620 and HCT15 cell lines
rapamycin no change compared to control in HCT15 cell line

T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PD0325901 decrease in SW620 and HCT15 cell lines
FMK-MEA no change compared to control in SW620 and HCT15 cell lines
PP242 increase slight increase in SW620 and HCT15 cell lines
PD0325901 PP242 inhibit treatment-induced increase
FMK-MEA PP242 no effect upon treatment-induced increase

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PD0325901 decrease in SW620 and HCT15 cell lines
FMK-MEA no change compared to control in SW620 and HCT15 cell lines
PP242 increase slight increase in SW620 and HCT15 cell lines
PD0325901 PP242 inhibit treatment-induced increase
FMK-MEA PP242 no effect upon treatment-induced increase

S2448-p - mTOR (human)
Modsite: RsRtRtDsysAGQsV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2448‑p, mTOR (mouse): S2448‑p, mTOR (rat): S2448‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP242 no change compared to control in SW620 and HCT15 cell lines

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KU-0063794 decrease in SW620 and HCT15 cell lines
INK-128 decrease in SW620 and HCT15 cell lines
PP242 decrease

S380-p - p90RSK (human)
Modsite: HQLFRGFsFVAtGLM SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): S380‑p, p90RSK iso2 (human): S389‑p, p90RSK (mouse): S369‑p, p90RSK iso3 (mouse): , p90RSK (rat): S380‑p, p90RSK (chicken): S398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP242 increase in SW620 and HCT15 cell lines
PD0325901 PP242 inhibit treatment-induced increase
FMK-MEA PP242 inhibit treatment-induced increase

S722-p - Raptor (human)
Modsite: PrLrsVssyGNIRAV SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S722‑p, Raptor (mouse): S722‑p, Raptor (rat): S722‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP242 increase in SW620 and HCT15 cell lines
PD0325901 PP242 inhibit treatment-induced increase
FMK-MEA PP242 inhibit treatment-induced increase

S240-p - S6 (human)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP242 decrease in SW620 HCT15 and SW48 cell lines
PD0325901 PP242 no effect upon treatment-induced decrease
FMK-MEA PP242 no effect upon treatment-induced decrease
KU-0063794 decrease in SW620 and HCT15 cell lines
INK-128 decrease in SW620 and HCT15 cell lines
rapamycin decrease
FMK-MEA increase

S244-p - S6 (human)
Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene
Orthologous residues
S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT15 (intestinal), SW48 (intestinal), SW620 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP242 decrease in SW620 HCT15 and SW48 cell lines
PD0325901 PP242 no effect upon treatment-induced decrease
FMK-MEA PP242 no effect upon treatment-induced decrease
KU-0063794 decrease in SW620 and HCT15 cell lines
INK-128 decrease in SW620 and HCT15 cell lines
rapamycin decrease
FMK-MEA increase