Taira J, Higashimoto Y (2014) Phosphorylation of Grb14 BPS domain by GSK-3 correlates with complex forming of Grb14 and insulin receptor. J Biochem 155, 353-60 24535599

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S358-p - GRB14 (human) ▲

Modsite: MHPYQGRsGCSsQSI SwissProt Entrez-Gene Orthologous residues GRB14 (human): S358‑p, GRB14 iso2 (human): S271‑p, GRB14 (mouse): S356‑p, GRB14 iso3 (mouse): ‑, GRB14 (rat): S356‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, microscopy-colocalization with upstream kinase, mutation of modification site Disease tissue studied:  liver cancer Relevant cell lines - cell types - tissues:  COS7 (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Enzymes shown to modify site in vitro:  Type Enzyme KINASE CK2A1 (human) KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) siRNA inhibition of enzyme, modification site within consensus motif, inhibition of upstream enzyme, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, phosphorylation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays INSR (human) BPS Disrupts co-immunoprecipitation Comments:  Phosphorylation after priming

S362-p - GRB14 (human) ▲

Modsite: QGRsGCSsQSIsPMR SwissProt Entrez-Gene Orthologous residues GRB14 (human): S362‑p, GRB14 iso2 (human): S275‑p, GRB14 (mouse): S360‑p, GRB14 iso3 (mouse): ‑, GRB14 (rat): S360‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, microscopy-colocalization with upstream kinase, mutation of modification site Disease tissue studied:  liver cancer Relevant cell lines - cell types - tissues:  COS7 (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, human Enzymes shown to modify site in vitro:  Type Enzyme KINASE CK2A1 (human) KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) siRNA inhibition of enzyme, modification site within consensus motif, inhibition of upstream enzyme, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, phosphorylation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays INSR (human) BPS Disrupts co-immunoprecipitation Comments:  Phosphorylation after priming

S366-p - GRB14 (human) ▲

Modsite: GCSsQSIsPMRsIsE SwissProt Entrez-Gene Orthologous residues GRB14 (human): S366‑p, GRB14 iso2 (human): S279‑p, GRB14 (mouse): S364‑p, GRB14 iso3 (mouse): ‑, GRB14 (rat): S364‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  liver cancer Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HepG2 (hepatic) Cellular systems studied:  cell lines Species studied:  green monkey, human Enzymes shown to modify site in vitro:  Type Enzyme KINASE CK2A1 (human) KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) siRNA inhibition of enzyme, modification site within consensus motif, inhibition of upstream enzyme, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin decrease GSK-3_inhibitor_IX decrease Downstream Regulation Effect of modification (function):  molecular association, regulation, phosphorylation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays INSR (human) BPS Disrupts co-immunoprecipitation Comments:  Phosphorylation after priming

S370-p - GRB14 (human) ▲
Modsite: QSIsPMRsIsENsLV SwissProt Entrez-Gene Orthologous residues GRB14 (human): S370‑p, GRB14 iso2 (human): S283‑p, GRB14 (mouse): S368‑p, GRB14 iso3 (mouse): ‑, GRB14 (rat): S368‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE CK2A1 (human) KINASE GSK3B (human)