Curated Information
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Home > Curated Information Page > PubMed Id: 24398676
Singh V, et al. (2014) AKT and GSK-3 Are Necessary for Direct Ezrin Binding to NHE3 as Part of a C-terminal Stimulatory Complex: ROLE OF A NOVEL SER-RICH NHE3 C-TERMINAL MOTIF IN NHE3 ACTIVITY AND TRAFFICKING. J Biol Chem 289, 5449-61 24398676
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S515-p - NHE3 (rabbit)
Modsite: NFDRRFLsKLLMRQS SwissProt Entrez-Gene
Orthologous residues
NHE3 (human): S516‑p, NHE3 (mouse): S511‑p, NHE3 (rat): S513‑p, NHE3 (rabbit): S515‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), OK (renal), PS120 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster, human, opossum
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt_inhibitor_VIII decrease
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Ezrin (human) Induces far-Western, in vitro

S522-p - NHE3 (rabbit)
Modsite: SKLLMRQsAQKSRDR SwissProt Entrez-Gene
Orthologous residues
NHE3 (human): S523‑p, NHE3 (mouse): S518‑p, NHE3 (rat): S520‑p, NHE3 (rabbit): S522‑p

S526-p - NHE3 (rabbit)
Modsite: MRQSAQKsRDRILNV SwissProt Entrez-Gene
Orthologous residues
NHE3 (human): S527‑p, NHE3 (mouse): S522‑p, NHE3 (rat): S524‑p, NHE3 (rabbit): S526‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SB216763 decrease
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization, molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Ezrin (human) Induces far-Western, in vitro
Comments:  stimulates Akt to phosphorylate S515