Curated Information
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Home > Curated Information Page > PubMed Id: 24403078
Lindsey-Boltz LA, et al. (2014) Coupling of human DNA excision repair and the DNA damage checkpoint in a defined in vitro system. J Biol Chem 289, 5074-82 24403078
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S345-p - Chk1 (mouse)
Modsite: LVQGISFsQPTCPEH SwissProt Entrez-Gene
Orthologous residues
Chk1 (human): S345‑p, Chk1 (mouse): S345‑p, Chk1 (rat): S345‑p, Chk1 (chicken): S345‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV no change compared to control
UV EXO1 (mouse) no change compared to control quiescent cells
UV increase
UV EXO1 (mouse) no effect upon treatment-induced increase

S18-p - p53 iso2 (mouse)
Modsite: ISLELPLsQETFSGL SwissProt Entrez-Gene
Orthologous residues
p53 (human): S15‑p, p53 iso2 (human): S15‑p, p53 iso4 (human): , p53 (mouse): S18‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (green monkey): S15‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATR (human)
Comments:  repair checkpoint coupling
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase quiescent cells
UV EXO1 (mouse) no change compared to control quiescent cells
UV increase
UV EXO1 (mouse) no effect upon treatment-induced increase

S33-p - RFA2 (mouse)
Modsite: GFGsPtPsQAEKKsR SwissProt Entrez-Gene
Orthologous residues
RFA2 (human): S33‑p, RFA2 iso3 (human): S121‑p, RFA2 (mouse): S33‑p, RFA2 (rat): S33‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATR (human)
Comments:  repair checkpoint coupling
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase quiescent cells
UV EXO1 (mouse) no change compared to control quiescent cells
UV increase
UV EXO1 (mouse) no effect upon treatment-induced increase