Curated Information
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Home > Curated Information Page > PubMed Id: 24270516
Chiang HS, et al. (2014) GEF-H1 controls microtubule-dependent sensing of nucleic acids for antiviral host defenses. Nat Immunol 15, 63-71 24270516
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y395-p - ARHGEF2 (mouse)
Modsite: VTQRITKyPVLINRI SwissProt Entrez-Gene
Orthologous residues
ARHGEF2 (human): Y394‑p, ARHGEF2 iso2 (human): Y393‑p, ARHGEF2 iso3 (human): Y366‑p, ARHGEF2 (mouse): Y395‑p, ARHGEF2 (rat): Y395‑p, ARHGEF2 (pig): Y368‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human

S885-p - ARHGEF2 (mouse)
Modsite: PLDPRRRsLPAGDAL SwissProt Entrez-Gene
Orthologous residues
ARHGEF2 (human): S886‑p, ARHGEF2 iso2 (human): S885‑p, ARHGEF2 iso3 (human): S858‑p, ARHGEF2 (mouse): S885‑p, ARHGEF2 (rat): S885‑p, ARHGEF2 (pig): S860‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase
colforsin increase
virus infection decrease NS1-deficient influenza A infection

S388-p - IRF3 (mouse)
Modsite: KTVDLHIsNSQPISL SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): S396‑p, IRF3 (mouse): S388‑p, IRF3 (rat): S390‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), macrophage-bone marrow
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
double-stranded_RNA, LPS ARHGEF2 (mouse) augment treatment-induced increase
double-stranded_RNA increase RIG-1 ligand 5'-ppp dsRNA

S534-p - NFkB-p65 (mouse)
Modsite: sGDEDFssIADMDFS SwissProt Entrez-Gene
Orthologous residues
NFkB‑p65 (human): S536‑p, NFkB‑p65 (mouse): S534‑p, NFkB‑p65 (rat): S535‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), macrophage-bone marrow
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
double-stranded_RNA, LPS ARHGEF2 (mouse) no effect upon treatment-induced increase
double-stranded_RNA increase RGI-1 5'-PPP dsRNA
virus infection increase NS1-deficient influenza A infection

Y701-p - STAT1 (mouse)
Modsite: DDPKRTGyIkTELIs SwissProt Entrez-Gene
Orthologous residues
STAT1 (human): Y701‑p, STAT1 iso2 (human): Y701‑p, STAT1 (mouse): Y701‑p, STAT1 (rat): Y701‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-bone marrow
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-beta increase