Curated Information
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Home > Curated Information Page > PubMed Id: 15896780
Sung KS, et al. (2005) Differential interactions of the homeodomain-interacting protein kinase 2 (HIPK2) by phosphorylation-dependent sumoylation. FEBS Lett 579, 3001-8 15896780
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K32-sm - HIPK2 (human)
Modsite: FCSVKKLkIEPSSNW SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): K32‑sm, HIPK2 (mouse): K32‑sm, HIPK2 (rat): K32‑sm
Characterization
Methods used to characterize site in vivo immunoassay, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DESUMOYLASE SENP1 (human) transfection of wild-type enzyme, transfection of inactive enzyme
SUMO LIGASE UBC9 (human) modification site within consensus motif, two-hybrid system, transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TLE1 (human) Disrupts pull-down assay