Novellasdemunt L, et al. (2013) PFKFB3 activation in cancer cells by the p38/MK2 pathway in response to stress stimuli. Biochem J 452, 531-43 23548149

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T334-p - MAPKAPK2 (human) ▲

Modsite: QstKVPQtPLHtsRV SwissProt Entrez-Gene Orthologous residues MAPKAPK2 (human): T334‑p, MAPKAPK2 iso2 (human): T334‑p, MAPKAPK2 (mouse): T320‑p, MAPKAPK2 (rat): T320‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes anisomycin increase

S461-p - PFKFB3 (human) ▲

Modsite: NPLMRRNsVtPLAsP SwissProt Entrez-Gene Orthologous residues PFKFB3 (human): S461‑p, PFKFB3 iso4 (human): S475‑p, PFKFB3 (mouse): S461‑p, PFKFB3 (rat): S490‑p Characterization Methods used to characterize site in vivo:  mass spectrometry (in vitro), multiple reaction monitoring (MRM), phospho-antibody, phosphopeptide mapping, western blotting Disease tissue studied:  glioblastoma multiforme, glioma, breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical), MCF-7 (breast cell), MEF (fibroblast), T98G (glial) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE MAPKAPK2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE MAPKAPK2 (human) transfection of constitutively active enzyme, genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme, activation of upstream enzyme, modification site within consensus motif, phospho-antibody, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes hypertonic_buffer increase anisomycin increase PF3644022 anisomycin inhibit treatment-induced increase PD98059 anisomycin, hypertonic_buffer no effect upon treatment-induced increase SB203580 anisomycin, hypertonic_buffer inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S478-p - PFKFB3 (human) ▲

Modsite: tKKPRINsFEEHVAS SwissProt Entrez-Gene Orthologous residues PFKFB3 (human): S478‑p, PFKFB3 iso4 (human): S492‑p, PFKFB3 (mouse): S478‑p, PFKFB3 (rat): S507‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes anisomycin no change compared to control

S103-p - SRF (human) ▲

Modsite: RGLKRsLsEMEIGMV SwissProt Entrez-Gene Orthologous residues SRF (human): S103‑p, SRF (mouse): S99‑p, SRF (rat): S99‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes anisomycin increase Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces