Curated Information
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Home > Curated Information Page > PubMed Id: 24297901
Nguyen le XT, Mitchell BS (2013) Akt activation enhances ribosomal RNA synthesis through casein kinase II and TIF-IA. Proc Natl Acad Sci U S A 110, 20681-6 24297901
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S129-p - Akt1 (human)
Modsite: sGsPsDNsGAEEMEV SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S129‑p, Akt1 iso2 (human): S67‑p, Akt1 (mouse): S129‑p, Akt1 (rat): S129‑p, Akt1 (fruit fly): E241‑p, Akt1 (cow): S129‑p

T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), leukocyte-blood
Cellular systems studied:  cell lines, primary cells
Species studied:  human

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  leukocyte-blood
Cellular systems studied:  cell lines, primary cells
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AZD8055 decrease

T13-p - CK2A1 (human)
Modsite: PsRARVytDVNtHRP SwissProt Entrez-Gene
Orthologous residues
CK2A1 (human): T13‑p, CK2A1 (mouse): T13‑p, CK2A1 (rat): T13‑p, CK2A1 (rabbit): T13‑p, CK2A1 (cow): T13‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) transfection of constitutively active enzyme, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, co-immunoprecipitation, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AZD8055 decrease
siRNA decrease siRNA for Akt1 inhibits phosphorylation

S170-p - TIF1A (human)
Modsite: KEGDVDVsDsDDEDD SwissProt Entrez-Gene
Orthologous residues
TIF1A (human): S170‑p, TIF1A (mouse): S168‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA decrease siRNA for CK2A1 decreases phosphorylation
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation, protein stabilization
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Induces co-immunoprecipitation
Comments:  rRNA synthesis induced

S172-p - TIF1A (human)
Modsite: GDVDVsDsDDEDDNL SwissProt Entrez-Gene
Orthologous residues
TIF1A (human): S172‑p, TIF1A (mouse): S170‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA decrease siRNA for CK2A1 decreases phosphorylation
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation, protein stabilization
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Induces co-immunoprecipitation
Comments:  rRNA synthesis induced