Curated Information
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Home > Curated Information Page > PubMed Id: 24308893
Illing S, Mann A, Schulz S (2014) Heterologous regulation of agonist-independent μ-opioid receptor phosphorylation by protein kinase C. Br J Pharmacol 171, 1330-40 24308893
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S363-p - MOR-1 (mouse)
Modsite: STIEQQNsARIRQNt SwissProt Entrez-Gene
Orthologous residues
MOR‑1 (human): S365‑p, MOR‑1 iso5 (human): S365‑p, MOR‑1 (mouse): S363‑p, MOR‑1 (rat): S363‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), brain [MOR-1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines, tissue
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCA (human) pharmacological inhibitor of upstream enzyme, phospho-antibody, pharmacological activator of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DAMGO no change compared to control
morphine no change compared to control
Ro-32-0432 decrease
etonitazene no change compared to control
etonitazene MOR-1 (mouse) increase MOR-1 -/- decrease
phorbol_ester no change compared to control

T370-p - MOR-1 (mouse)
Modsite: sARIRQNtREHPstA SwissProt Entrez-Gene
Orthologous residues
MOR‑1 (human): T372‑p, MOR‑1 iso5 (human): T372‑p, MOR‑1 (mouse): T370‑p, MOR‑1 (rat): T370‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCA (human) pharmacological inhibitor of upstream enzyme, phospho-antibody, pharmacological activator of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DAMGO increase
phorbol_ester increase
phorbol_ester increase
BIM23627 phorbol_ester inhibit treatment-induced increase
Ro-32-0432 phorbol_ester inhibit treatment-induced increase
LY333531 phorbol_ester inhibit treatment-induced increase
H-89 phorbol_ester no effect upon treatment-induced increase
GRK2_inhibitor no effect upon treatment-induced increase
phorbol_ester PKCA (mouse) increase PKCA siRNA inhibits
phorbol_ester PKCB (mouse) no effect upon treatment-induced increase
PKCB iso2 (mouse) no change compared to control
phorbol_ester PKCG (mouse) no effect upon treatment-induced increase
phorbol_ester PKCE (mouse) no effect upon treatment-induced increase
morphine no change compared to control
Ro-32-0432 no change compared to control

S375-p - MOR-1 (mouse)
Modsite: QNtREHPstANtVDR SwissProt Entrez-Gene
Orthologous residues
MOR‑1 (human): S377‑p, MOR‑1 iso5 (human): S377‑p, MOR‑1 (mouse): S375‑p, MOR‑1 (rat): S375‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), brain [MOR-1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines, tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DAMGO increase
phorbol_ester no change compared to control
morphine increase
Ro-32-0432 no change compared to control
etonitazene increase
etonitazene MOR-1 (mouse) increase MOR-1 -/- decrease

T376-p - MOR-1 (mouse)
Modsite: NtREHPstANtVDRT SwissProt Entrez-Gene
Orthologous residues
MOR‑1 (human): T378‑p, MOR‑1 iso5 (human): T378‑p, MOR‑1 (mouse): T376‑p, MOR‑1 (rat): T376‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DAMGO increase
phorbol_ester no change compared to control

T379-p - MOR-1 (mouse)
Modsite: EHPstANtVDRTNHQ SwissProt Entrez-Gene
Orthologous residues
MOR‑1 (human): T381‑p, MOR‑1 iso5 (human): T381‑p, MOR‑1 (mouse): T379‑p, MOR‑1 (rat): T379‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DAMGO increase
phorbol_ester no change compared to control