Curated Information
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Home > Curated Information Page > PubMed Id: 24362026
Wang H, et al. (2014) The CUL7/F-box and WD Repeat Domain Containing 8 (CUL7/Fbxw8) Ubiquitin Ligase Promotes Degradation of Hematopoietic Progenitor Kinase 1. J Biol Chem 289, 4009-17 24362026
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T165-p - HPK1 (human)
Modsite: ISAQIGAtLARRLsF SwissProt Entrez-Gene
Orthologous residues
HPK1 (human): T165‑p, HPK1 (mouse): T165‑p, HPK1 (rat): T165‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, protein degradation

S171-p - HPK1 (human)
Modsite: AtLARRLsFIGtPyW SwissProt Entrez-Gene
Orthologous residues
HPK1 (human): S171‑p, HPK1 (mouse): S171‑p, HPK1 (rat): S171‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, protein degradation

T175-p - HPK1 (human)
Modsite: RRLsFIGtPyWMAPE SwissProt Entrez-Gene
Orthologous residues
HPK1 (human): T175‑p, HPK1 (mouse): T175‑p, HPK1 (rat): T175‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, protein degradation

T349-p - HPK1 (human)
Modsite: RKLRGMEtRPPANtA SwissProt Entrez-Gene
Orthologous residues
HPK1 (human): T349‑p, HPK1 (mouse): C348‑p, HPK1 (rat): C348‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

T355-p - HPK1 (human)
Modsite: EtRPPANtARLQPPR SwissProt Entrez-Gene
Orthologous residues
HPK1 (human): T355‑p, HPK1 (mouse): T354‑p, HPK1 (rat): T354‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP4C (human) pharmacological inhibitor of upstream enzyme, transfection of inactive enzyme
Downstream Regulation
Effect of modification (function):  ubiquitination