Chung HH, Sze SK, Tay AS, Lin VC (2014) Acetylation at Lysine 183 of Progesterone Receptor by p300 Accelerates DNA Binding Kinetics and Transactivation of Direct Target Genes. J Biol Chem 289, 2180-94 24302725

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K183-ac - PR (human) ▲

Modsite: SGTAAAHkVLPRGLs SwissProt Entrez-Gene Orthologous residues PR (human): K183‑ac, PR iso2 (human): K19‑ac, PR (mouse): K184‑ac, PR (rat): K183‑ac, PR (chicken): P146‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HeLa (cervical), MDA-MB-231 (breast cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) co-immunoprecipitation, transfection of wild-type enzyme, siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes trichostatin_A, progestin_R5020 increase progestin_R5020 no change compared to control trichostatin_A no change compared to control Downstream Regulation Effect of modification (function):  molecular association, regulation, phosphorylation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces co-immunoprecipitation Comments:  FKBP5 transcription; associated with increased S294 phosphorylation

S294-p - PR (human) ▲

Modsite: APMAPGRsPLATTVM SwissProt Entrez-Gene Orthologous residues PR (human): S294‑p, PR iso2 (human): S130‑p, PR (mouse): S294‑p, PR (rat): S293‑p, PR (chicken): G219‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes trichostatin_A, progestin_R5020 increase progestin_R5020 no change compared to control trichostatin_A no change compared to control