Wu CF, et al. (2014) RSK promotes G2/M transition through activating phosphorylation of Cdc25A and Cdc25B. Oncogene 33, 2385-94 23708659

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S293-p - CDC25A (human) ▲

Modsite: GSTKrRKsMsGASPk SwissProt Entrez-Gene Orthologous residues CDC25A (human): S293‑p, CDC25A iso2 (human): S253‑p, CDC25A (mouse): S284‑p, CDC25A (rat): S296‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  293 (epithelial), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE p90RSK (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE p90RSK (human) phospho-antibody, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes BI-D1870 decrease SL0101 decrease

S295-p - CDC25A (human) ▲

Modsite: TKrRKsMsGASPkES SwissProt Entrez-Gene Orthologous residues CDC25A (human): S295‑p, CDC25A iso2 (human): S255‑p, CDC25A (mouse): P286‑p, CDC25A (rat): S297‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  293 (epithelial), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE p90RSK (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE p90RSK (human) phospho-antibody, pharmacological inhibitor of upstream enzyme

S353-p - CDC25B (human) ▲

Modsite: VQNkRRRsVtPPEEQ SwissProt Entrez-Gene Orthologous residues CDC25B (human): S353‑p, CDC25B iso2 (human): S339‑p, CDC25B iso3 (human): S312‑p, CDC25B (mouse): S351‑p, CDC25B (rat): S349‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  293 (epithelial), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE p90RSK (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE p90RSK (human) phospho-antibody, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes BI-D1870 decrease SL0101 decrease

T355-p - CDC25B (human) ▲

Modsite: NkRRRsVtPPEEQQE SwissProt Entrez-Gene Orthologous residues CDC25B (human): T355‑p, CDC25B iso2 (human): T341‑p, CDC25B iso3 (human): T314‑p, CDC25B (mouse): T353‑p, CDC25B (rat): T351‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  293 (epithelial), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE p90RSK (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE p90RSK (human) phospho-antibody, pharmacological inhibitor of upstream enzyme

S247-p - CDC25C (human) ▲

Modsite: kVKKkYFsGQGkLRK SwissProt Entrez-Gene Orthologous residues CDC25C (human): S247‑p, CDC25C iso3 (human): S204‑p, CDC25C (mouse): ‑, CDC25C (frog): T318‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  293 (epithelial), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE p90RSK (human)

T359-p - p90RSK (human) ▲

Modsite: DTEFTSRtPKDsPGI SwissProt Entrez-Gene Orthologous residues p90RSK (human): T359‑p, p90RSK iso2 (human): T368‑p, p90RSK (mouse): T348‑p, p90RSK iso3 (mouse): ‑, p90RSK (rat): T359‑p, p90RSK (chicken): T377‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  293 (epithelial), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S363-p - p90RSK (human) ▲

Modsite: TSRtPKDsPGIPPsA SwissProt Entrez-Gene Orthologous residues p90RSK (human): S363‑p, p90RSK iso2 (human): S372‑p, p90RSK (mouse): S352‑p, p90RSK iso3 (mouse): ‑, p90RSK (rat): S363‑p, p90RSK (chicken): S381‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  293 (epithelial), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S227-p - RSK2 (human) ▲

Modsite: DHEKKAysFCGtVEy SwissProt Entrez-Gene Orthologous residues RSK2 (human): S227‑p, RSK2 (mouse): S227‑p, RSK2 (rat): S227‑p, RSK2 (cow): S227‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  293 (epithelial), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  enzymatic activity, induced