Guthridge MA, et al. (2004) The phosphoserine-585-dependent pathway of the GM-CSF/IL-3/IL-5 receptors mediates hematopoietic cell survival through activation of NF-kappaB and induction of bcl-2. Blood 103, 820-7 12920017

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T308-p - Akt1 (human) ▲

Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene Orthologous residues Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  CTL-EN (T lymphocyte) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes CSF2RB (human) increase

S473-p - Akt1 (human) ▲

Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  CTL-EN (T lymphocyte) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes CSF2RB (human) increase

Y593-p - CSF2RB (human) ▲

Modsite: SFDFNGPyLGPPHSR SwissProt Entrez-Gene Orthologous residues CSF2RB (human): Y593‑p, CSF2RB iso2 (human): Y599‑p, CSF2RB (mouse): Y595‑p, CSF2RB iso2 (mouse): Y595‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  CTL-EN (T lymphocyte) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes GM-CSF increase

S601-p - CSF2RB (human) ▲

Modsite: LGPPHSRsLPDILGQ SwissProt Entrez-Gene Orthologous residues CSF2RB (human): S601‑p, CSF2RB iso2 (human): S607‑p, CSF2RB (mouse): S603‑p, CSF2RB iso2 (mouse): S603‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  CTL-EN (T lymphocyte) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes GM-CSF increase SB203580 GM-CSF no effect upon treatment-induced increase PD90059 GM-CSF no effect upon treatment-induced increase LY294002 GM-CSF inhibit treatment-induced increase AG490 GM-CSF inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  activity, induced, molecular association, regulation, phosphorylation Effect of modification (process):  apoptosis, inhibited, transcription, altered Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays 14-3-3 beta (human) Induces molecular association, regulation co-immunoprecipitation PIK3R1 (human) Induces molecular association, regulation co-immunoprecipitation Comments:  S601 is important for NF-kB and Akt activation, and Bcl-2 expression.