Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 18593906
Morishita D, et al. (2008) Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels. Cancer Res 68, 5076-85 18593906
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T32-p - FOXO3A (human)
Modsite: QsRPRsCtWPLQRPE SwissProt Entrez-Gene
Orthologous residues
FOXO3A (human): T32‑p, FOXO3A (mouse): T32‑p, FOXO3A (rat): T32‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pim1 (human) co-immunoprecipitation, transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  activity, inhibited
Effect of modification (process):  transcription, altered

S253-p - FOXO3A (human)
Modsite: APRRRAVsMDNSNkY SwissProt Entrez-Gene
Orthologous residues
FOXO3A (human): S253‑p, FOXO3A (mouse): S252‑p, FOXO3A (rat): S252‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pim1 (human) co-immunoprecipitation, transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  activity, inhibited
Effect of modification (process):  transcription, altered

T157-p - p27Kip1 (human)
Modsite: GIRkrPAtDDSSTQN SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T157‑p, p27Kip1 (mouse): A157‑p, p27Kip1 (rat): A157‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody
Disease tissue studied:  leukemia, chronic myelogenous leukemia, prostate cancer
Relevant cell lines - cell types - tissues:  22Rv1 (prostate cell), HEK293T (epithelial), K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Pim1 (human)
Comments:  Pim1 short isoform (Pim1S)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pim1 (human) co-immunoprecipitation, transfection of inactive enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation, protein degradation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 theta (human) Induces co-immunoprecipitation

T198-p - p27Kip1 (human)
Modsite: PGLRRRQt_______ SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody
Disease tissue studied:  leukemia, chronic myelogenous leukemia, prostate cancer
Relevant cell lines - cell types - tissues:  22Rv1 (prostate cell), HEK293T (epithelial), K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Pim1 (human)
Comments:  Pim1 short isoform (Pim1S)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pim1 (human) co-immunoprecipitation, transfection of inactive enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation, protein degradation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 theta (human) Induces co-immunoprecipitation