Kruljac-Letunic A, et al. (2003) The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1. J Biol Chem 278, 29560-70 12771146

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Y308-p - DDEF1 (mouse) ▲

Modsite: PKEVGGLyVASRANS SwissProt Entrez-Gene Orthologous residues DDEF1 (human): ‑, DDEF1 iso2 (human): ‑, DDEF1 (mouse): Y308‑p, DDEF1 (rat): Y308‑p, DDEF1 (cow): ‑ Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Pyk2 (mouse) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Src iso1 (mouse) transfection of dominant-negative enzyme, transfection of wild-type enzyme KINASE Pyk2 (mouse) transfection of dominant-negative enzyme, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  activity, inhibited, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Pyk2 (mouse) Induces in vitro, affinity chromatography Comments:  modulates DDEF1 phospholipid binding as well as ARF1 GTPase activity

Y782-p - DDEF1 (mouse) ▲

Modsite: RDKQRLSyGAFTNQI SwissProt Entrez-Gene Orthologous residues DDEF1 (human): Y767‑p, DDEF1 iso2 (human): Y770‑p, DDEF1 (mouse): Y782‑p, DDEF1 (rat): Y779‑p, DDEF1 (cow): Y770‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  activity, inhibited Comments:  modulates DDEF1 phospholipid binding as well as ARF1 GTPase activity

Y402-p - Pyk2 (mouse) ▲

Modsite: CSIESDIyAEIPDET SwissProt Entrez-Gene Orthologous residues Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human