Thévenin AF, et al. (2017) Phosphorylation regulates connexin43/ZO-1 binding and release, an important step in gap junction turnover. Mol Biol Cell 28, 3595-3608 29021339

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S255-p - GJA1 (rat) ▲

Modsite: HATTGPLsPskDCGs SwissProt Entrez-Gene Orthologous residues GJA1 (human): S255‑p, GJA1 (mouse): S255‑p, GJA1 (rat): S255‑p, GJA1 (rabbit): S255‑p, GJA1 (pig): S255‑p, GJA1 (hamster): N255‑p, GJA1 (cow): S256‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical), MDCK (epithelial) Cellular systems studied:  cell lines Species studied:  dog, human

S279-p - GJA1 (rat) ▲

Modsite: SSPTAPLsPMsPPGY SwissProt Entrez-Gene Orthologous residues GJA1 (human): S279‑p, GJA1 (mouse): S279‑p, GJA1 (rat): S279‑p, GJA1 (rabbit): S279‑p, GJA1 (pig): S279‑p, GJA1 (hamster): S279‑p, GJA1 (cow): S280‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical), MDCK (epithelial) Cellular systems studied:  cell lines Species studied:  dog, human

S282-p - GJA1 (rat) ▲

Modsite: TAPLsPMsPPGYkLV SwissProt Entrez-Gene Orthologous residues GJA1 (human): S282‑p, GJA1 (mouse): S282‑p, GJA1 (rat): S282‑p, GJA1 (rabbit): S282‑p, GJA1 (pig): S282‑p, GJA1 (hamster): S282‑p, GJA1 (cow): S283‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical), MDCK (epithelial) Cellular systems studied:  cell lines Species studied:  dog, human

S365-p - GJA1 (rat) ▲

Modsite: IVDQRPssRAssRAs SwissProt Entrez-Gene Orthologous residues GJA1 (human): S365‑p, GJA1 (mouse): S365‑p, GJA1 (rat): S365‑p, GJA1 (rabbit): S365‑p, GJA1 (pig): S365‑p, GJA1 (hamster): S365‑p, GJA1 (cow): S366‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical), MDCK (epithelial) Cellular systems studied:  cell lines Species studied:  dog, human Downstream Regulation Effect of modification (function):  activity, induced Comments:  channel open, (S373 dephosphorylated)

S368-p - GJA1 (rat) ▲

Modsite: QRPssRAssRAssRP SwissProt Entrez-Gene Orthologous residues GJA1 (human): S368‑p, GJA1 (mouse): S368‑p, GJA1 (rat): S368‑p, GJA1 (rabbit): S368‑p, GJA1 (pig): S368‑p, GJA1 (hamster): S368‑p, GJA1 (cow): S369‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical), MDCK (epithelial) Cellular systems studied:  cell lines Species studied:  dog, human Downstream Regulation Effect of modification (function):  activity, inhibited Comments:  channel closed (S365 dephosphorylated)

S373-p - GJA1 (rat) ▲

Modsite: RAssRAssRPRPDDL SwissProt Entrez-Gene Orthologous residues GJA1 (human): S373‑p, GJA1 (mouse): S373‑p, GJA1 (rat): S373‑p, GJA1 (rabbit): S373‑p, GJA1 (pig): S373‑p, GJA1 (hamster): S373‑p, GJA1 (cow): S374‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical), MDCK (epithelial) Cellular systems studied:  cell lines Species studied:  dog, human Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays ZO1 (rat) Disrupts co-immunoprecipitation Comments:  transport to the plasma membrane