Curated Information
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Home > Curated Information Page > PubMed Id: 24247720
Shin S, Wolgamott L, Roux PP, Yoon SO (2014) Casein kinase 1ε promotes cell proliferation by regulating mRNA translation. Cancer Res 74, 201-11 24247720
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T37-p - 4E-BP1 (human)
Modsite: PPGDysttPGGtLFs SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p, 4E‑BP1 (cow): T37‑p
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  T41 and T50 phosphorylation is required for stepwise phosphorylation of T37, T46, S65, and S70

T41-p - 4E-BP1 (human)
Modsite: ysttPGGtLFsttPG SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T41‑p, 4E‑BP1 (mouse): T40‑p, 4E‑BP1 (rat): T40‑p, 4E‑BP1 (fruit fly): T41‑p, 4E‑BP1 (cow): T41‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1E (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1E (human) siRNA inhibition of enzyme, modification site within consensus motif, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

T46-p - 4E-BP1 (human)
Modsite: GGtLFsttPGGtRII SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p, 4E‑BP1 (cow): T46‑p
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  T41 and T50 phosphorylation is required for stepwise phosphorylation of T37, T46, S65, and S70

T50-p - 4E-BP1 (human)
Modsite: FsttPGGtRIIyDRk SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T50‑p, 4E‑BP1 (mouse): T49‑p, 4E‑BP1 (rat): T49‑p, 4E‑BP1 (fruit fly): T50‑p, 4E‑BP1 (cow): T50‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1E (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1E (human) siRNA inhibition of enzyme, modification site within consensus motif, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S65-p - 4E-BP1 (human)
Modsite: FLMECrNsPVtktPP SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): S65‑p, 4E‑BP1 (mouse): S64‑p, 4E‑BP1 (rat): S64‑p, 4E‑BP1 (fruit fly): S65‑p, 4E‑BP1 (cow): S65‑p
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  T41 and T50 phosphorylation is required for stepwise phosphorylation of T37, T46, S65, and S70

T70-p - 4E-BP1 (human)
Modsite: rNsPVtktPPRDLPt SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T70‑p, 4E‑BP1 (mouse): T69‑p, 4E‑BP1 (rat): T69‑p, 4E‑BP1 (fruit fly): T70‑p, 4E‑BP1 (cow): T70‑p
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  T41 and T50 phosphorylation is required for stepwise phosphorylation of T37, T46, S65, and S70

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CK1E (human) no change compared to control CK1E shRNA no effect
PF-670462 no change compared to control

S209-p - EIF4E (human)
Modsite: DtATksGsttKNRFV SwissProt Entrez-Gene
Orthologous residues
EIF4E (human): S209‑p, EIF4E iso2 (human): S240‑p, EIF4E (mouse): S209‑p, EIF4E (rat): S209‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CK1E (human) no change compared to control CK1E shRNA no effect
PF-670462 no change compared to control

T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CK1E (human) no change compared to control CK1E shRNA no effect
PF-670462 no change compared to control

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CK1E (human) no change compared to control CK1E shRNA no effect
PF-670462 no change compared to control

T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CK1E (human) no change compared to control CK1E shRNA no effect
PF-670462 no change compared to control

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CK1E (human) no change compared to control CK1E shRNA no effect
PF-670462 no change compared to control

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CK1E (human) no change compared to control CK1E shRNA no effect
PF-670462 no change compared to control

T246-p - PRAS40 (human)
Modsite: LPRPRLNtsDFQkLK SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso2 (human): T116‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p, PRAS40 (cow): T246‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CK1E (human) no change compared to control CK1E shRNA no effect
PF-670462 no change compared to control

S792-p - Raptor (human)
Modsite: DKMRRAssYSsLNSL SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S792‑p, Raptor (mouse): S792‑p, Raptor (rat): S792‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CK1E (human) no change compared to control CK1E shRNA no effect
PF-670462 no change compared to control