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Home > Curated Information Page > PubMed Id: 24247980
Treebak JT, et al. (2014) Acute exercise and physiological insulin induce distinct phosphorylation signatures on TBC1D1 and TBC1D4 proteins in human skeletal muscle. J Physiol 592, 351-75 24247980
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S80-p - ACC1 (human)
Modsite: LHIRssMsGLHLVkQ SwissProt Entrez-Gene
Orthologous residues
ACC1 (human): S80‑p, ACC1 iso2 (human): S22‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p

S222-p - ACC2 (human)
Modsite: PtMRPsMsGLHLVKR SwissProt Entrez-Gene
Orthologous residues
ACC2 (human): S222‑p, ACC2 (mouse): S212‑p, ACC2 (rat): S218‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase

T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
meal feeding, exercise increase
exercise, fasting no change compared to control

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rest increase
meal feeding rest augment treatment-induced increase
exercise, fasting increase

T172-p - AMPKA2 (human)
Modsite: sDGEFLRtsCGsPNy SwissProt Entrez-Gene
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p, AMPKA2 (pig): T172‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase

S318-p - AS160 (human)
Modsite: EFRsRCssVtGVQRR SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S318‑p, AS160 iso2 (human): S318‑p, AS160 iso3 (human): S318‑p, AS160 (mouse): S324‑p, AS160 iso2 (mouse): S324‑p, AS160 (rat): S326‑p, AS160 (cow): S319‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S341-p - AS160 (human)
Modsite: QPRRRHAsAPsHVQP SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S341‑p, AS160 iso2 (human): S341‑p, AS160 iso3 (human): S341‑p, AS160 (mouse): S348‑p, AS160 iso2 (mouse): S348‑p, AS160 (rat): S350‑p, AS160 (cow): S343‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase
insulin increase
insulin, exercise increase

S588-p - AS160 (human)
Modsite: RMRGRLGsVDsFERs SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S588‑p, AS160 iso2 (human): S588‑p, AS160 iso3 (human): S588‑p, AS160 (mouse): S595‑p, AS160 iso2 (mouse): S595‑p, AS160 (rat): S597‑p, AS160 (cow): S590‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase
insulin no change compared to control

T642-p - AS160 (human)
Modsite: QFRRRAHtFsHPPss SwissProt Entrez-Gene
Orthologous residues
AS160 (human): T642‑p, AS160 iso2 (human): T642‑p, AS160 iso3 (human): T642‑p, AS160 (mouse): T649‑p, AS160 iso2 (mouse): T649‑p, AS160 (rat): T651‑p, AS160 (cow): T644‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase
insulin increase
insulin, exercise increase
muscle contraction decrease

S666-p - AS160 (human)
Modsite: GRAQGVRsPLLRQSS SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S666‑p, AS160 iso2 (human): S666‑p, AS160 iso3 (human): S666‑p, AS160 (mouse): S673‑p, AS160 iso2 (mouse): S673‑p, AS160 (rat): S675‑p, AS160 (cow): S668‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
exercise no change compared to control

S704-p - AS160 (human)
Modsite: PSLHtSFsAPsFTAP SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S704‑p, AS160 iso2 (human): , AS160 iso3 (human): S704‑p, AS160 (mouse): S711‑p, AS160 iso2 (mouse): , AS160 (rat): S713‑p, AS160 (cow): S706‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase
insulin increase
insulin, exercise increase
muscle contraction increase
muscle contraction AMPKA2 (human) augment treatment-induced increase

S751-p - AS160 (human)
Modsite: EGRKRtsstCsNEsL SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S751‑p, AS160 iso2 (human): S688‑p, AS160 iso3 (human): S743‑p, AS160 (mouse): S758‑p, AS160 iso2 (mouse): S695‑p, AS160 (rat): S760‑p, AS160 (cow): S753‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase
insulin no change compared to control
muscle contraction decrease

S237-p - TBC1D1 (human)
Modsite: RPMRKsFsQPGLRSL SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S237‑p, TBC1D1 iso2 (human): S237‑p, TBC1D1 (mouse): S231‑p, TBC1D1 iso2 (mouse): S231‑p, TBC1D1 (rat): S231‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase
insulin no change compared to control
muscle contraction increase

T596-p - TBC1D1 (human)
Modsite: AFRRRANtLsHFPIE SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): T596‑p, TBC1D1 iso2 (human): T596‑p, TBC1D1 (mouse): T590‑p, TBC1D1 iso2 (mouse): T590‑p, TBC1D1 (rat): T590‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA2 (human) phospho-motif antibody, transfection of wild-type enzyme, activation of upstream enzyme, genetic knockout/knockin of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase
insulin increase
insulin, exercise increase
muscle contraction increase
siRNA decrease siRNA for AMPK2 decreases phosphorylation

S324-p - AS160 (mouse)
Modsite: EFRsRCssVTGVMQK SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S318‑p, AS160 iso2 (human): S318‑p, AS160 iso3 (human): S318‑p, AS160 (mouse): S324‑p, AS160 iso2 (mouse): S324‑p, AS160 (rat): S326‑p, AS160 (cow): S319‑p

S348-p - AS160 (mouse)
Modsite: QPRRRHAsAPsHVQP SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S341‑p, AS160 iso2 (human): S341‑p, AS160 iso3 (human): S341‑p, AS160 (mouse): S348‑p, AS160 iso2 (mouse): S348‑p, AS160 (rat): S350‑p, AS160 (cow): S343‑p

S595-p - AS160 (mouse)
Modsite: RMRGRLGsMDsFERA SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S588‑p, AS160 iso2 (human): S588‑p, AS160 iso3 (human): S588‑p, AS160 (mouse): S595‑p, AS160 iso2 (mouse): S595‑p, AS160 (rat): S597‑p, AS160 (cow): S590‑p

T649-p - AS160 (mouse)
Modsite: QFRRRAHtFsHPPSS SwissProt Entrez-Gene
Orthologous residues
AS160 (human): T642‑p, AS160 iso2 (human): T642‑p, AS160 iso3 (human): T642‑p, AS160 (mouse): T649‑p, AS160 iso2 (mouse): T649‑p, AS160 (rat): T651‑p, AS160 (cow): T644‑p

S673-p - AS160 (mouse)
Modsite: GKAHGLRsPLLRQSs SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S666‑p, AS160 iso2 (human): S666‑p, AS160 iso3 (human): S666‑p, AS160 (mouse): S673‑p, AS160 iso2 (mouse): S673‑p, AS160 (rat): S675‑p, AS160 (cow): S668‑p

S711-p - AS160 (mouse)
Modsite: PSLHTSFsAPSFTAP SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S704‑p, AS160 iso2 (human): , AS160 iso3 (human): S704‑p, AS160 (mouse): S711‑p, AS160 iso2 (mouse): , AS160 (rat): S713‑p, AS160 (cow): S706‑p

S758-p - AS160 (mouse)
Modsite: EGRKRtsstCsNEsL SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S751‑p, AS160 iso2 (human): S688‑p, AS160 iso3 (human): S743‑p, AS160 (mouse): S758‑p, AS160 iso2 (mouse): S695‑p, AS160 (rat): S760‑p, AS160 (cow): S753‑p

S231-p - TBC1D1 (mouse)
Modsite: RPMRKsFsQPGLRSL SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S237‑p, TBC1D1 iso2 (human): S237‑p, TBC1D1 (mouse): S231‑p, TBC1D1 iso2 (mouse): S231‑p, TBC1D1 (rat): S231‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase
insulin no change compared to control
muscle contraction decrease

T590-p - TBC1D1 (mouse)
Modsite: AFRRRANtLsHFPVE SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): T596‑p, TBC1D1 iso2 (human): T596‑p, TBC1D1 (mouse): T590‑p, TBC1D1 iso2 (mouse): T590‑p, TBC1D1 (rat): T590‑p

S660-p - TBC1D1 (mouse)
Modsite: PRLNPSAssPNFFKY SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): , TBC1D1 iso2 (human): S667‑p, TBC1D1 (mouse): S660‑p, TBC1D1 iso2 (mouse): , TBC1D1 (rat): S662‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase
insulin no change compared to control
muscle contraction increase

S700-p - TBC1D1 (mouse)
Modsite: KKLHssssVPNFLKF SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): , TBC1D1 iso2 (human): S707‑p, TBC1D1 (mouse): S700‑p, TBC1D1 iso2 (mouse): , TBC1D1 (rat): S702‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
exercise increase
insulin no change compared to control
muscle contraction increase