Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.4
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 24145406
Bitomsky N, et al. (2013) Autophosphorylation and Pin1 binding coordinate DNA damage-induced HIPK2 activation and cell death. Proc Natl Acad Sci U S A 110, E4203-12 24145406
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S139-p - H2AX (human)
Modsite: GkkAtQAsQEy____ SwissProt Entrez-Gene
Orthologous residues
H2AX (human): S139‑p, H2AX (mouse): S139‑p, H2AX (rat): S139‑p
Characterization
Methods used to characterize site in vivo immunoassay, phospho-antibody
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
adriamycin increase

S668-p - HIPK2 (human)
Modsite: GFQGLQAsPSkHAGY SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): S668‑p, HIPK2 (mouse): S668‑p, HIPK2 (rat): S668‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
KINASE HIPK2 (human)
Comments:  Autophosphorylation

S827-p - HIPK2 (human)
Modsite: SSSQAIssPQRSKRV SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): S827‑p, HIPK2 (mouse): S827‑p, HIPK2 (rat): S826‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
KINASE HIPK2 (human)
Comments:  Autophosphorylation

T838-p - HIPK2 (human)
Modsite: SKRVKENtPPRCAMV SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): T838‑p, HIPK2 (mouse): T838‑p, HIPK2 (rat): T837‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
KINASE HIPK2 (human)
Comments:  Autophosphorylation

S848-p - HIPK2 (human)
Modsite: RCAMVHSsPACSTSV SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): S848‑p, HIPK2 (mouse): S848‑p, HIPK2 (rat): S847‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
KINASE HIPK2 (human)
Comments:  Autophosphorylation

T880-p - HIPK2 (human)
Modsite: QTIVIPDtPsPTVSV SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): T880‑p, HIPK2 (mouse): T880‑p, HIPK2 (rat): T879‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human, zebrafish
Enzymes shown to modify site in vitro
Type Enzyme
KINASE HIPK2 (human)
Comments:  Autophosphorylation
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE HIPK2 (human)
Comments:  Autophosphorylation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
adriamycin increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, phosphorylation, protein conformation, protein stabilization
Effect of modification (process):  apoptosis, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIN1 (human) Induces co-immunoprecipitation, pull-down assay

S882-p - HIPK2 (human)
Modsite: IVIPDtPsPTVSVIT SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): S882‑p, HIPK2 (mouse): S882‑p, HIPK2 (rat): S881‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human, zebrafish
Enzymes shown to modify site in vitro
Type Enzyme
KINASE HIPK2 (human)
Comments:  Autophosphorylation
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE HIPK2 (human)
Comments:  Autophosphorylation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
adriamycin increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, phosphorylation, protein conformation, protein stabilization
Effect of modification (process):  apoptosis, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIN1 (human) Induces co-immunoprecipitation, pull-down assay

S924-p - HIPK2 (human)
Modsite: SCVTVHDsPYSDSSS SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): S924‑p, HIPK2 (mouse): S924‑p, HIPK2 (rat): S923‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
KINASE HIPK2 (human)
Comments:  Autophosphorylation

S934-p - HIPK2 (human)
Modsite: SDSSSNTsPYSVQQR SwissProt Entrez-Gene
Orthologous residues
HIPK2 (human): S934‑p, HIPK2 (mouse): S934‑p, HIPK2 (rat): S933‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
KINASE HIPK2 (human)
Comments:  Autophosphorylation

S46-p - p53 (human)
Modsite: AMDDLMLsPDDIEQW SwissProt Entrez-Gene
Orthologous residues
p53 (human): S46‑p, p53 iso2 (human): S46‑p, p53 iso4 (human): S7‑p, p53 (mouse): , p53 iso2 (mouse): , p53 (rat): , p53 (rabbit): S45‑p, p53 (green monkey): S46‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PIN1 (human) increase
HIPK2 (human) increase