Bitomsky N, et al. (2013) Autophosphorylation and Pin1 binding coordinate DNA damage-induced HIPK2 activation and cell death. Proc Natl Acad Sci U S A 110, E4203-12 24145406

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S139-p - H2AX (human) ▲

Modsite: GkkAtQAsQEy____ SwissProt Entrez-Gene Orthologous residues H2AX (human): S139‑p, H2AX (mouse): S139‑p, H2AX (rat): S139‑p Characterization Methods used to characterize site in vivo:  immunoassay, phospho-antibody Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  U2OS (bone cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes adriamycin increase

S668-p - HIPK2 (human) ▲
Modsite: GFQGLQAsPSkHAGY SwissProt Entrez-Gene Orthologous residues HIPK2 (human): S668‑p, HIPK2 (mouse): S668‑p, HIPK2 (rat): S668‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE HIPK2 (human) Comments:  Autophosphorylation

S827-p - HIPK2 (human) ▲
Modsite: SSSQAIssPQRSKRV SwissProt Entrez-Gene Orthologous residues HIPK2 (human): S827‑p, HIPK2 (mouse): S827‑p, HIPK2 (rat): S826‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE HIPK2 (human) Comments:  Autophosphorylation

T838-p - HIPK2 (human) ▲
Modsite: SKRVKENtPPRCAMV SwissProt Entrez-Gene Orthologous residues HIPK2 (human): T838‑p, HIPK2 (mouse): T838‑p, HIPK2 (rat): T837‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE HIPK2 (human) Comments:  Autophosphorylation

S848-p - HIPK2 (human) ▲
Modsite: RCAMVHSsPACSTSV SwissProt Entrez-Gene Orthologous residues HIPK2 (human): S848‑p, HIPK2 (mouse): S848‑p, HIPK2 (rat): S847‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE HIPK2 (human) Comments:  Autophosphorylation

T880-p - HIPK2 (human) ▲

Modsite: QTIVIPDtPsPTVSV SwissProt Entrez-Gene Orthologous residues HIPK2 (human): T880‑p, HIPK2 (mouse): T880‑p, HIPK2 (rat): T879‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma, lung cancer, non-small cell lung cancer Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human, zebrafish Enzymes shown to modify site in vitro:  Type Enzyme KINASE HIPK2 (human) Comments:  Autophosphorylation Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE HIPK2 (human) Comments:  Autophosphorylation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes adriamycin increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, phosphorylation, protein conformation, protein stabilization Effect of modification (process):  apoptosis, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PIN1 (human) Induces co-immunoprecipitation, pull-down assay

S882-p - HIPK2 (human) ▲

Modsite: IVIPDtPsPTVSVIT SwissProt Entrez-Gene Orthologous residues HIPK2 (human): S882‑p, HIPK2 (mouse): S882‑p, HIPK2 (rat): S881‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma, lung cancer, non-small cell lung cancer Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human, zebrafish Enzymes shown to modify site in vitro:  Type Enzyme KINASE HIPK2 (human) Comments:  Autophosphorylation Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE HIPK2 (human) Comments:  Autophosphorylation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes adriamycin increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, phosphorylation, protein conformation, protein stabilization Effect of modification (process):  apoptosis, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PIN1 (human) Induces co-immunoprecipitation, pull-down assay

S924-p - HIPK2 (human) ▲
Modsite: SCVTVHDsPYSDSSS SwissProt Entrez-Gene Orthologous residues HIPK2 (human): S924‑p, HIPK2 (mouse): S924‑p, HIPK2 (rat): S923‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE HIPK2 (human) Comments:  Autophosphorylation

S934-p - HIPK2 (human) ▲
Modsite: SDSSSNTsPYSVQQR SwissProt Entrez-Gene Orthologous residues HIPK2 (human): S934‑p, HIPK2 (mouse): S934‑p, HIPK2 (rat): S933‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE HIPK2 (human) Comments:  Autophosphorylation

S46-p - p53 (human) ▲

Modsite: AMDDLMLsPDDIEQW SwissProt Entrez-Gene Orthologous residues p53 (human): S46‑p, p53 iso2 (human): S46‑p, p53 iso4 (human): S7‑p, p53 (mouse): ‑, p53 iso2 (mouse): ‑, p53 (rat): ‑, p53 (rabbit): S45‑p, p53 (green monkey): S46‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PIN1 (human) increase HIPK2 (human) increase