Crewe C, Kinter M, Szweda LI (2013) Rapid inhibition of pyruvate dehydrogenase: an initiating event in high dietary fat-induced loss of metabolic flexibility in the heart. PLoS One 8, e77280 24116221

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T308-p - Akt1 (mouse) ▲

Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene Orthologous residues Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  heart [PDHK4 (mouse), homozygous knockout] Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase high-fat diet insulin inhibit treatment-induced increase high-fat diet, insulin PDHK4 (mouse) decrease PDK4 KO-no effect on treatment induced increase

S232-p - PDHA1 (mouse) ▲

Modsite: NRyGMGtsVERAAAS SwissProt Entrez-Gene Orthologous residues PDHA1 (human): S232‑p, PDHA1 iso2 (human): S239‑p, PDHA1 iso4 (human): S270‑p, PDHA1 (mouse): S232‑p, PDHA1 (rat): S232‑p, PDHA1 (pig): S231‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  heart Cellular systems studied:  tissue Species studied:  mouse Comments:  mitochondria from hearts of mice fed a high-fat diet Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase S232 showed greatest increase.

S293-p - PDHA1 (mouse) ▲

Modsite: TYRyHGHsMsDPGVs SwissProt Entrez-Gene Orthologous residues PDHA1 (human): S293‑p, PDHA1 iso2 (human): S300‑p, PDHA1 iso4 (human): S331‑p, PDHA1 (mouse): S293‑p, PDHA1 (rat): S293‑p, PDHA1 (pig): S292‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  heart Cellular systems studied:  tissue Species studied:  mouse Comments:  mitochondria from hearts of mice fed a high-fat diet Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase S232 showed greatest increase.

S300-p - PDHA1 (mouse) ▲

Modsite: sMsDPGVsyRtREEI SwissProt Entrez-Gene Orthologous residues PDHA1 (human): S300‑p, PDHA1 iso2 (human): S307‑p, PDHA1 iso4 (human): S338‑p, PDHA1 (mouse): S300‑p, PDHA1 (rat): S300‑p, PDHA1 (pig): S299‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  heart Cellular systems studied:  tissue Species studied:  mouse Comments:  mitochondria from hearts of mice fed a high-fat diet Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase S232 showed greatest increase.