Stiffel V, et al. (2014) EphA4 receptor is a novel negative regulator of osteoclast activity. J Bone Miner Res 29, 804-19 23983218

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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Y319-p - EFNB2 (mouse) ▲

Modsite: GDyGHPVyIVQEMPP SwissProt Entrez-Gene Orthologous residues EFNB2 (human): Y316‑p, EFNB2 (mouse): Y319‑p, EFNB2 (rat): Y319‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  osteoclast, RAW 264.7 (macrophage) Cellular systems studied:  cell lines, primary cultured cells Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EphA4 (mouse) no change compared to control siRNA

Y772-p - ITGB3 (mouse) ▲

Modsite: DtANNPLykEATStF SwissProt Entrez-Gene Orthologous residues ITGB3 (human): Y773‑p, ITGB3 iso2 (human): ‑, ITGB3 (mouse): Y772‑p, ITGB3 (rat): Y772‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  osteoclast Cellular systems studied:  primary cultured cells Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EphA4 (mouse) decrease Epha4-null EphA4 (mouse) decrease Epha4-fc EFNA4 (mouse) increase EFNA4-fc Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays DOK1 (mouse) Induces co-immunoprecipitation Comments:  inhibits beta 3 integrin signaling

Y784-p - ITGB3 (mouse) ▲

Modsite: StFTNITyRGT____ SwissProt Entrez-Gene Orthologous residues ITGB3 (human): Y785‑p, ITGB3 iso2 (human): ‑, ITGB3 (mouse): Y784‑p, ITGB3 (rat): Y784‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  osteoclast Cellular systems studied:  primary cultured cells Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EphA4 (mouse) decrease Epha4 null

Y527-p - Src iso1 (mouse) ▲

Modsite: LQAFLEDyFTSTEPQ SwissProt Entrez-Gene Orthologous residues Src (human): Y522‑p, Src iso2 (human): Y528‑p, Src (mouse): Y521‑p, Src iso1 (mouse): Y527‑p, Src (rat): Y522‑p, Src (chicken): Y519‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  osteoclast Cellular systems studied:  primary cultured cells Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EphA4 (mouse) decrease Epha4-fc EFNA4 (mouse) decrease EFNA4-fc EphA4 (mouse) no change compared to control Epha4 null osteclast

Y173-p - VAV3 (mouse) ▲

Modsite: EDEGGEVyEDLMKAE SwissProt Entrez-Gene Orthologous residues VAV3 (human): Y173‑p, VAV3 iso4 (human): Y173‑p, VAV3 (mouse): Y173‑p, VAV3 (rat): Y173‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  osteoclast Cellular systems studied:  primary cultured cells Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EphA4 (mouse) increase Epha4-null osteoclast increase EphA4 (mouse) increase Epha4-fc EFNA4 (mouse) decrease EFNA4-fc decrease