Martinez LO, et al. (2003) Phosphorylation of a pest sequence in ABCA1 promotes calpain degradation and is reversed by ApoA-I. J Biol Chem 278, 37368-74 12869555

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T1286-p - ABCA1 (mouse) ▲

Modsite: QSCLHPFtEDDAVDP SwissProt Entrez-Gene Orthologous residues ABCA1 (human): T1286‑p, ABCA1 iso100 (human): T1286‑p, ABCA1 (mouse): T1286‑p, ABCA1 (rat): T1226‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), macrophage-peritoneum Cellular systems studied:  cell lines, primary cells Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes apoA-I decrease apigenin no change compared to control H-89 decrease Downstream Regulation Effect of modification (function):  protein degradation, protein processing Comments:  Mutants showed increase of cell surface protein levels., T1286A and T1305A mutants are not degraded by calpain or stabilized by apoA-1.

T1305-p - ABCA1 (mouse) ▲

Modsite: IDPESREtDLLSGMD SwissProt Entrez-Gene Orthologous residues ABCA1 (human): T1305‑p, ABCA1 iso100 (human): T1305‑p, ABCA1 (mouse): T1305‑p, ABCA1 (rat): T1245‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), macrophage-peritoneum Cellular systems studied:  cell lines, primary cells Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes apoA-I decrease apigenin no change compared to control H-89 decrease Downstream Regulation Effect of modification (function):  protein degradation, protein processing Comments:  Mutants showed increase of cell surface protein levels., T1286A and T1305A mutants are not degraded by calpain or stabilized by apoA-1.