Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 23950959
Simon S, et al. (2013) Analysis of the Dominant Effects Mediated by Wild Type or R120G Mutant of αB-crystallin (HspB5) towards Hsp27 (HspB1). PLoS One 8, e70545 23950959
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S19-p - CRYAB (human)
Modsite: RPFFPFHsPsrLFDQ SwissProt Entrez-Gene
Orthologous residues
CRYAB (human): S19‑p, CRYAB (mouse): S19‑p, CRYAB (rat): S19‑p, CRYAB (rabbit): S19‑p, CRYAB (pig): S19‑p, CRYAB (cow): S19‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
menadione increase
menadione increase
mutation menadione augment treatment-induced increase R120G
Downstream Regulation
Effect of modification (function):  protein conformation, protein stabilization
Comments:  regulates cell morphology and oxidoresistance

S45-p - CRYAB (human)
Modsite: FPTSTsLsPFYLrPP SwissProt Entrez-Gene
Orthologous residues
CRYAB (human): S45‑p, CRYAB (mouse): S45‑p, CRYAB (rat): S45‑p, CRYAB (rabbit): S45‑p, CRYAB (pig): S45‑p, CRYAB (cow): S45‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
menadione increase
menadione increase
mutation menadione augment treatment-induced increase R120G
Downstream Regulation
Effect of modification (function):  protein conformation, protein stabilization
Comments:  regulates cell morphology and oxidoresistance

S59-p - CRYAB (human)
Modsite: PsFLRAPsWFDTGLS SwissProt Entrez-Gene
Orthologous residues
CRYAB (human): S59‑p, CRYAB (mouse): S59‑p, CRYAB (rat): S59‑p, CRYAB (rabbit): S59‑p, CRYAB (pig): S59‑p, CRYAB (cow): S59‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
menadione increase
menadione increase
mutation menadione augment treatment-induced increase R120G
Downstream Regulation
Effect of modification (function):  protein conformation, protein stabilization
Comments:  regulates cell morphology and oxidoresistance

S15-p - HSP27 (human)
Modsite: FsLLrGPsWDPFrDW SwissProt Entrez-Gene
Orthologous residues
HSP27 (human): S15‑p, HSP27 (mouse): S15‑p, HSP27 (rat): S15‑p, HSP27 (pig): S15‑p, HSP27 (hamster): S15‑p, HSP27 (chicken): S15‑p, HSP27 (dog): S15‑p, HSP27 (cow): S15‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein conformation, protein stabilization
Comments:  regulates cell morphology and oxidoresistance

S78-p - HSP27 (human)
Modsite: PAysRALsRQLssGV SwissProt Entrez-Gene
Orthologous residues
HSP27 (human): S78‑p, HSP27 (mouse): N82‑p, HSP27 (rat): N82‑p, HSP27 (pig): S80‑p, HSP27 (hamster): N86‑p, HSP27 (chicken): S77‑p, HSP27 (dog): S82‑p, HSP27 (cow): S74‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein conformation, protein stabilization
Comments:  regulates cell morphology and oxidoresistance

S82-p - HSP27 (human)
Modsite: RALsRQLssGVsEIr SwissProt Entrez-Gene
Orthologous residues
HSP27 (human): S82‑p, HSP27 (mouse): S86‑p, HSP27 (rat): S86‑p, HSP27 (pig): S84‑p, HSP27 (hamster): S90‑p, HSP27 (chicken): S80‑p, HSP27 (dog): S86‑p, HSP27 (cow): S78‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein conformation, protein stabilization
Comments:  regulates cell morphology and oxidoresistance