Simon S, et al. (2013) Analysis of the Dominant Effects Mediated by Wild Type or R120G Mutant of αB-crystallin (HspB5) towards Hsp27 (HspB1). PLoS One 8, e70545 23950959

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S19-p - CRYAB (human) ▲

Modsite: RPFFPFHsPsrLFDQ SwissProt Entrez-Gene Orthologous residues CRYAB (human): S19‑p, CRYAB (mouse): S19‑p, CRYAB (rat): S19‑p, CRYAB (rabbit): S19‑p, CRYAB (pig): S19‑p, CRYAB (cow): S19‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes menadione increase mutation menadione augment treatment-induced increase R120G

S45-p - CRYAB (human) ▲

Modsite: FPTSTsLsPFYLrPP SwissProt Entrez-Gene Orthologous residues CRYAB (human): S45‑p, CRYAB (mouse): S45‑p, CRYAB (rat): S45‑p, CRYAB (rabbit): S45‑p, CRYAB (pig): S45‑p, CRYAB (cow): S45‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes menadione increase mutation menadione augment treatment-induced increase R120G

S59-p - CRYAB (human) ▲

Modsite: PsFLRAPsWFDTGLS SwissProt Entrez-Gene Orthologous residues CRYAB (human): S59‑p, CRYAB (mouse): S59‑p, CRYAB (rat): S59‑p, CRYAB (rabbit): S59‑p, CRYAB (pig): S59‑p, CRYAB (cow): S59‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes menadione increase mutation menadione augment treatment-induced increase R120G

S15-p - HSP27 (human) ▲

Modsite: FsLLrGPsWDPFrDW SwissProt Entrez-Gene Orthologous residues HSP27 (human): S15‑p, HSP27 (mouse): S15‑p, HSP27 (rat): S15‑p, HSP27 (pig): S15‑p, HSP27 (hamster): S15‑p, HSP27 (chicken): S15‑p, HSP27 (dog): S15‑p, HSP27 (cow): S15‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes menadione no change compared to control mutation menadione increase R120G Downstream Regulation Effect of modification (function):  protein stabilization Comments:  stabilize HSP27 interaction with mutant CRYAB

S78-p - HSP27 (human) ▲

Modsite: PAysRALsRQLssGV SwissProt Entrez-Gene Orthologous residues HSP27 (human): S78‑p, HSP27 (mouse): N82‑p, HSP27 (rat): N82‑p, HSP27 (pig): S80‑p, HSP27 (hamster): N86‑p, HSP27 (chicken): S77‑p, HSP27 (dog): S82‑p, HSP27 (cow): S74‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes menadione no change compared to control mutation menadione increase R120G

S82-p - HSP27 (human) ▲

Modsite: RALsRQLssGVsEIr SwissProt Entrez-Gene Orthologous residues HSP27 (human): S82‑p, HSP27 (mouse): S86‑p, HSP27 (rat): S86‑p, HSP27 (pig): S84‑p, HSP27 (hamster): S90‑p, HSP27 (chicken): S80‑p, HSP27 (dog): S86‑p, HSP27 (cow): S78‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes menadione no change compared to control mutation menadione increase R120G