Curated Information
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Home > Curated Information Page > PubMed Id: 23905686
Liu Y, et al. (2013) Phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) is an AMPK target participating in contraction-stimulated glucose uptake in skeletal muscle. Biochem J 455, 195-206 23905686
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S202-p - ACC2 (mouse)
Modsite: LNTsDPEsHAPtMRP SwissProt Entrez-Gene
Orthologous residues
ACC2 (human): T212‑p, ACC2 (mouse): S202‑p, ACC2 (rat): S208‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 293T (epithelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PIKFYVE (mouse) no change compared to control PIKfyve siRNA no change
insulin no change compared to control
PIKFYVE (mouse) no change compared to control PIKFYVE siRNA no change
acadesine increase
PIKFYVE (mouse) no effect upon treatment-induced increase PIKFYVE siRNA

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 293T (epithelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PIKFYVE (mouse) no change compared to control PIKfyve siRNA no change
insulin increase
insulin PIKFYVE (mouse) no change compared to control PIKFYVE siRNA no change
acadesine no change compared to control
acadesine PIKFYVE (human) no change compared to control PIKFYVE siRNA no change

T183-p - AMPKA1 (mouse)
Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 293T (epithelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PIKFYVE (mouse) no change compared to control PIKfyve siRNA no change
insulin no change compared to control
PIKFYVE (mouse) no change compared to control PIKFYVE siRNA no change
acadesine increase
PIKFYVE (mouse) no effect upon treatment-induced increase PIKFYVE siRNA

S48-p - PIKFYVE (mouse)
Modsite: PFKSAYSsFVNLFRF SwissProt Entrez-Gene
Orthologous residues
PIKFYVE (human): S48‑p, PIKFYVE iso2 (human): S48‑p, PIKFYVE iso6 (human): , PIKFYVE (mouse): S48‑p, PIKFYVE iso1 (mouse): S48‑p, PIKFYVE iso3 (mouse): S48‑p, PIKFYVE (rat): S48‑p, PIKFYVE iso2 (rat): S48‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 293T (epithelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (mouse)
KINASE Akt1 (mouse)

S307-p - PIKFYVE (mouse)
Modsite: PARNRsAsItNLsLD SwissProt Entrez-Gene
Orthologous residues
PIKFYVE (human): S307‑p, PIKFYVE iso2 (human): S210‑p, PIKFYVE iso6 (human): , PIKFYVE (mouse): S307‑p, PIKFYVE iso1 (mouse): S318‑p, PIKFYVE iso3 (mouse): S307‑p, PIKFYVE (rat): S318‑p, PIKFYVE iso2 (rat): S307‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 293T (epithelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (mouse)
KINASE AMPKA1 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (mouse) transfection of dominant-negative enzyme, activation of upstream enzyme, transfection of wild-type enzyme, phospho-antibody
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  recruitment to vesicles to catalyse PtdIns(3,5)P2 production

S792-p - Raptor (mouse)
Modsite: DKMRRVSsYSALNSL SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S792‑p, Raptor (mouse): S792‑p, Raptor (rat): S792‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 293T (epithelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PIKFYVE (mouse) no change compared to control PIKfyve siRNA no change
insulin no change compared to control
PIKFYVE (mouse) no change compared to control PIKFYVE siRNA no change
acadesine increase
PIKFYVE (mouse) no effect upon treatment-induced increase PIKFYVE siRNA

T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 293T (epithelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 293T (epithelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat

S9-p - GSK3B (rat)
Modsite: SGRPRTTsFAESCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 293T (epithelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat