Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 23897807
Ayoub E, et al. (2013) Regulation of the Src kinase-associated phosphoprotein 55 homologue by the protein tyrosine phosphatase PTP-PEST in the control of cell motility. J Biol Chem 288, 25739-48 23897807
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S39-p - PTP-PEST (mouse)
Modsite: FMRLRRLsTKYRTEK SwissProt Entrez-Gene
Orthologous residues
PTP‑PEST (human): S39‑p, PTP‑PEST iso2 (human): , PTP‑PEST iso3 (human): , PTP‑PEST (mouse): S39‑p, PTP‑PEST (rat): S39‑p, PTP‑PEST (cow): S39‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), MEF (fibroblast) [SKAP2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SKAP2 (mouse) SH3 Disrupts co-immunoprecipitation

S434-p - PTP-PEST (mouse)
Modsite: KKLERNLsFEIKKVP SwissProt Entrez-Gene
Orthologous residues
PTP‑PEST (human): S435‑p, PTP‑PEST iso2 (human): S305‑p, PTP‑PEST iso3 (human): S316‑p, PTP‑PEST (mouse): S434‑p, PTP‑PEST (rat): S434‑p, PTP‑PEST (cow): S435‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), MEF (fibroblast) [SKAP2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SKAP2 (mouse) SH3 Induces co-immunoprecipitation

Y260-p - SKAP2 (mouse)
Modsite: QPIDDEIyEELPEEE SwissProt Entrez-Gene
Orthologous residues
SKAP2 (human): Y261‑p, SKAP2 (mouse): Y260‑p, SKAP2 (rat): Y260‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), MEF (fibroblast) [SKAP2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PTP-PEST (mouse) genetic knockout/knockin of upstream enzyme, co-immunoprecipitation, transfection of wild-type enzyme

Y297-p - SKAP2 (mouse)
Modsite: SGDKSTDyANFYQGL SwissProt Entrez-Gene
Orthologous residues
SKAP2 (human): Y298‑p, SKAP2 (mouse): Y297‑p, SKAP2 (rat): Y297‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), MEF (fibroblast) [SKAP2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PTP-PEST (mouse) genetic knockout/knockin of upstream enzyme, co-immunoprecipitation, transfection of wild-type enzyme