Curated Information
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Home > Curated Information Page > PubMed Id: 23833262
Yang SS, et al. (2013) Phosphorylation regulates NCC stability and transporter activity in vivo. J Am Soc Nephrol 24, 1587-97 23833262
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T53-p - NCCT (mouse)
Modsite: GstLyMRtFGYNtID SwissProt Entrez-Gene
Orthologous residues
NCCT (human): T55‑p, NCCT iso2 (human): T55‑p, NCCT iso3 (human): T55‑p, NCCT (mouse): T53‑p, NCCT (rat): T53‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  kidney
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
STLK3 (mouse) increase
low-salt diet increase
WNK4 (mouse) increase D561A mutant

T58-p - NCCT (mouse)
Modsite: MRtFGYNtIDVVPAY SwissProt Entrez-Gene
Orthologous residues
NCCT (human): T60‑p, NCCT iso2 (human): T60‑p, NCCT iso3 (human): T60‑p, NCCT (mouse): T58‑p, NCCT (rat): T58‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  kidney, MDCK (epithelial), renal
Cellular systems studied:  cell lines, primary cells, primary cultured cells, tissue
Species studied:  dog, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE STLK3 (mouse) phospho-antibody, activation of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
low-salt diet increase
WNK4 (mouse) increase D561A mutant
Downstream Regulation
Effect of modification (function):  intracellular localization, phosphorylation, protein stabilization
Associated Diseases
Diseases Alterations Comments
GS mutation of site

S71-p - NCCT (mouse)
Modsite: AYEHYANsALPGEPR SwissProt Entrez-Gene
Orthologous residues
NCCT (human): S73‑p, NCCT iso2 (human): S73‑p, NCCT iso3 (human): S73‑p, NCCT (mouse): S71‑p, NCCT (rat): S71‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  kidney
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
STLK3 (mouse) increase
low-salt diet increase
WNK4 (mouse) increase D561A mutant