Curated Information
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Home > Curated Information Page > PubMed Id: 23778885
Li Y, Huang X, Zhang Q, Ma K (2013) Phosphorylation of cMet tyrosine residues in murine ascitic hepatic cancer cell lines with different lymph node metastatic potentials. Mol Med Rep 8, 655-61 23778885
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  Mouse hepatoma ascites cell line- Hca-F (high metastatic potential) & Hca-P (Low metastatic potential)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HGF increase
fibronectin no change compared to control
laminin-1 no change compared to control not different between Hca-P and Hca-F cells

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  Mouse hepatoma ascites cell line- Hca-F (high metastatic potential) & Hca-P (Low metastatic potential)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HGF increase
fibronectin no change compared to control
laminin-1 no change compared to control not different between Hca-P and Hca-F cells

Y1313-p - Met (human)
Modsite: EyCPDPLyEVMLkCW SwissProt Entrez-Gene
Orthologous residues
Met (human): Y1313‑p, Met iso2 (human): Y1331‑p, Met (mouse): Y1311‑p, Met (rat): Y1314‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  Mouse hepatoma ascites cell line- Hca-F (high metastatic potential) & Hca-P (Low metastatic potential)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HGF increase
fibronectin increase
laminin-1 no change compared to control not different between Hca-P and Hca-F cells

Y1349-p - Met (human)
Modsite: stFIGEHyVHVNAty SwissProt Entrez-Gene
Orthologous residues
Met (human): Y1349‑p, Met iso2 (human): Y1367‑p, Met (mouse): Y1347‑p, Met (rat): Y1350‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  Mouse hepatoma ascites cell line- Hca-F (high metastatic potential) & Hca-P (Low metastatic potential)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin no change compared to control
laminin-1 no change compared to control not different between Hca-P and Hca-F cells
increase higher in Hca-P cells than in Hca-F cells

Y1365-p - Met (human)
Modsite: NVKCVAPyPsLLssE SwissProt Entrez-Gene
Orthologous residues
Met (human): Y1365‑p, Met iso2 (human): Y1383‑p, Met (mouse): Y1363‑p, Met (rat): Y1366‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  Mouse hepatoma ascites cell line- Hca-F (high metastatic potential) & Hca-P (Low metastatic potential)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HGF increase
fibronectin no change compared to control
laminin-1 increase

Y783-p - PLCG1 (human)
Modsite: EGRNPGFyVEANPMP SwissProt Entrez-Gene
Orthologous residues
PLCG1 (human): Y783‑p, PLCG1 iso2 (human): Y783‑p, PLCG1 (mouse): Y783‑p, PLCG1 (rat): Y783‑p, PLCG1 (cow): Y783‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  Mouse hepatoma ascites cell line- Hca-F (high metastatic potential) & Hca-P (Low metastatic potential)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HGF increase
fibronectin increase
laminin-1 increase