Curated Information
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Home > Curated Information Page > PubMed Id: 16806191
Stafford MJ, Morrice NA, Peggie MW, Cohen P (2006) Interleukin-1 stimulated activation of the COT catalytic subunit through the phosphorylation of Thr290 and Ser62. FEBS Lett 580, 4010-4 16806191
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S62-p - Cot (human)
Modsite: QNDERSKsLLLSGQE SwissProt Entrez-Gene
Orthologous residues
Cot (human): S62‑p, Cot (mouse): S62‑p, Cot (rat): S62‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), Sf21
Cellular systems studied:  cell lines
Species studied:  human, insect
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S125-p - Cot (human)
Modsite: NGRYQIDsDVLLIPW SwissProt Entrez-Gene
Orthologous residues
Cot (human): S125‑p, Cot (mouse): S125‑p, Cot (rat): S125‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Sf21
Cellular systems studied:  cell lines
Species studied:  insect

S141-p - Cot (human)
Modsite: LTyRNIGsDFIPRGA SwissProt Entrez-Gene
Orthologous residues
Cot (human): S141‑p, Cot (mouse): S141‑p, Cot (rat): S141‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Sf21
Cellular systems studied:  cell lines
Species studied:  insect

T290-p - Cot (human)
Modsite: FPKDLRGtEIYMSPE SwissProt Entrez-Gene
Orthologous residues
Cot (human): T290‑p, Cot (mouse): T290‑p, Cot (rat): T290‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
PS-1145 serum no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Comments:  required for phosphorylation of Cot Ser62

S334-p - Cot (human)
Modsite: WVKRYPRsAYPSYLY SwissProt Entrez-Gene
Orthologous residues
Cot (human): S334‑p, Cot (mouse): S334‑p, Cot (rat): S334‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Sf21
Cellular systems studied:  cell lines
Species studied:  insect