Curated Information
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Home > Curated Information Page > PubMed Id: 9391119
Giordano S, et al. (1997) A point mutation in the MET oncogene abrogates metastasis without affecting transformation. Proc Natl Acad Sci U S A 94, 13868-72 9391119
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y1349-p - Met (human)
Modsite: stFIGEHyVHVNAty SwissProt Entrez-Gene
Orthologous residues
Met (human): Y1349‑p, Met iso2 (human): Y1367‑p, Met (mouse): Y1347‑p, Met (rat): Y1350‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  Fr 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  carcinogenesis, induced, cell growth, altered, cell motility, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GRB2 (human) SH2 Induces molecular association, regulation cell growth, altered, cell motility, altered functional assay
Comments:  Double mutant Y1349F/Y1356F inhibits both metastasis and transformation. N1358H is also critical for malignant transformation as it affects Grb2 binding and activation of the Ras pathway (mutantGRB-). H1352N which makes an high affinity GRB2 was highly transforming, but not metastatic (mutant 2X Grb2). Expression of the recombinant oncogene TPR-SEA, was comaparable to 2X GRB2 mutant phenotype. A GRB2/2X GRB2 mutant was rescued by complementation in trans and resumed the ability to invade an in vitro reconstituted basement membrane.

Y1356-p - Met (human)
Modsite: yVHVNAtyVNVKCVA SwissProt Entrez-Gene
Orthologous residues
Met (human): Y1356‑p, Met iso2 (human): Y1374‑p, Met (mouse): Y1354‑p, Met (rat): Y1357‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  Fr 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  carcinogenesis, induced, cell growth, altered, cell motility, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GRB2 (human) SH2 Induces molecular association, regulation cell growth, altered, cell motility, altered functional assay
Comments:  Double mutant Y1349F/Y1356F inhibits both metastasis and transformation. N1358H is also critical for malignant transformation as it affects Grb2 binding and activation of the Ras pathway (mutantGRB-). H1352N which makes an high affinity GRB2 was highly transforming, but not metastatic (mutant 2X Grb2). Expression of the recombinant oncogene TPR-SEA, was comaparable to 2X GRB2 mutant phenotype. A GRB2/2X GRB2 mutant was rescued by complementation in trans and resumed the ability to invade an in vitro reconstituted basement membrane.