Horiike Y, Kobayashi H, Sekiguchi T (2009) Ran GTPase guanine nucleotide exchange factor RCC1 is phosphorylated on serine 11 by cdc2 kinase in vitro. Mol Biol Rep 36, 717-23 18568422

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S2-p - RCC1 (human) ▲

Modsite: ______MsPKRIAKR SwissProt Entrez-Gene Orthologous residues RCC1 (human): S2‑p, RCC1 iso2 (human): S2‑p, RCC1 (mouse): P2‑p, RCC1 (rat): P2‑p Characterization Methods used to characterize site in vivo:  2D analysis, electrophoretic mobility shift, mutation of modification site Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster

S11-p - RCC1 (human) ▲

Modsite: KRIAKRRsPPADAIP SwissProt Entrez-Gene Orthologous residues RCC1 (human): S11‑p, RCC1 iso2 (human): S11‑p, RCC1 (mouse): S11‑p, RCC1 (rat): S11‑p Characterization Methods used to characterize site in vivo:  2D analysis, electrophoretic mobility shift, mutation of modification site Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human) Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cytoskeletal reorganization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays KPNA4 (human) Disrupts co-immunoprecipitation

S20-p - RCC1 (human) ▲

Modsite: PADAIPKsKKVKVsH SwissProt Entrez-Gene Orthologous residues RCC1 (human): S20‑p, RCC1 iso2 (human): S20‑p, RCC1 (mouse): S20‑p, RCC1 (rat): S20‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays KPNA4 (human) Disrupts co-immunoprecipitation

S26-p - RCC1 (human) ▲

Modsite: KsKKVKVsHRsHStE SwissProt Entrez-Gene Orthologous residues RCC1 (human): S26‑p, RCC1 iso2 (human): S57‑p, RCC1 (mouse): S26‑p, RCC1 (rat): S26‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays KPNA4 (human) Disrupts co-immunoprecipitation

S29-p - RCC1 (human) ▲

Modsite: KVKVsHRsHStEPGL SwissProt Entrez-Gene Orthologous residues RCC1 (human): S29‑p, RCC1 iso2 (human): S60‑p, RCC1 (mouse): S29‑p, RCC1 (rat): S29‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays KPNA4 (human) Disrupts co-immunoprecipitation

T32-p - RCC1 (human) ▲

Modsite: VsHRsHStEPGLVLT SwissProt Entrez-Gene Orthologous residues RCC1 (human): T32‑p, RCC1 iso2 (human): T63‑p, RCC1 (mouse): T32‑p, RCC1 (rat): T32‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays KPNA4 (human) Disrupts co-immunoprecipitation

T274-p - RCC1 (human) ▲
Modsite: SNYHQLGtPGTESCF SwissProt Entrez-Gene Orthologous residues RCC1 (human): T274‑p, RCC1 iso2 (human): T305‑p, RCC1 (mouse): T274‑p, RCC1 (rat): T274‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human)

S387-p - RCC1 (human) ▲
Modsite: GQDEDAWsPVEMMGK SwissProt Entrez-Gene Orthologous residues RCC1 (human): S387‑p, RCC1 iso2 (human): S418‑p, RCC1 (mouse): S387‑p, RCC1 (rat): S387‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human)