Curated Information
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Home > Curated Information Page > PubMed Id: 18545697
Jelluma N, et al. (2008) Chromosomal instability by inefficient Mps1 auto-activation due to a weakened mitotic checkpoint and lagging chromosomes. PLoS One 3, e2415 18545697
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T33-p - TTK (human)
Modsite: kFkNEDLtDELsLNK SwissProt Entrez-Gene
Orthologous residues
TTK (human): T33‑p, TTK iso2 (human): T33‑p, TTK (mouse): T32‑p, TTK iso2 (mouse): T32‑p, TTK (rat): T32‑p, TTK (frog): T34‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S37-p - TTK (human)
Modsite: EDLtDELsLNKISAD SwissProt Entrez-Gene
Orthologous residues
TTK (human): S37‑p, TTK iso2 (human): S37‑p, TTK (mouse): S36‑p, TTK iso2 (mouse): S36‑p, TTK (rat): N36‑p, TTK (frog): T38‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S80-p - TTK (human)
Modsite: EkNSVPLsDALLNkL SwissProt Entrez-Gene
Orthologous residues
TTK (human): S80‑p, TTK iso2 (human): S80‑p, TTK (mouse): N76‑p, TTK iso2 (mouse): N76‑p, TTK (rat): N76‑p, TTK (frog): D81‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

T360-p - TTK (human)
Modsite: sITLKNktEssLLAk SwissProt Entrez-Gene
Orthologous residues
TTK (human): T360‑p, TTK iso2 (human): T360‑p, TTK (mouse): T380‑p, TTK iso2 (mouse): T354‑p, TTK (rat): T355‑p, TTK (frog): Q388‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S363-p - TTK (human)
Modsite: LKNktEssLLAkLEE SwissProt Entrez-Gene
Orthologous residues
TTK (human): S363‑p, TTK iso2 (human): S363‑p, TTK (mouse): S383‑p, TTK iso2 (mouse): S357‑p, TTK (rat): S358‑p, TTK (frog): S391‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S393-p - TTK (human)
Modsite: QWQSKRksECINQNP SwissProt Entrez-Gene
Orthologous residues
TTK (human): S393‑p, TTK iso2 (human): S393‑p, TTK (mouse): P408‑p, TTK iso2 (mouse): P382‑p, TTK (rat): P387‑p, TTK (frog): S409‑p

S436-p - TTK (human)
Modsite: VFSVskQsPPIsTSk SwissProt Entrez-Gene
Orthologous residues
TTK (human): S436‑p, TTK iso2 (human): S435‑p, TTK (mouse): S435‑p, TTK iso2 (mouse): S409‑p, TTK (rat): S414‑p, TTK (frog): S459‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

Y462-p - TTK (human)
Modsite: sSNtLDDyMsCFrtP SwissProt Entrez-Gene
Orthologous residues
TTK (human): Y462‑p, TTK iso2 (human): Y461‑p, TTK (mouse): Y461‑p, TTK iso2 (mouse): Y435‑p, TTK (rat): Y440‑p, TTK (frog): Y485‑p

T676-p - TTK (human)
Modsite: NQMQPDttsVVkDSQ SwissProt Entrez-Gene
Orthologous residues
TTK (human): T676‑p, TTK iso2 (human): T675‑p, TTK (mouse): T675‑p, TTK iso2 (mouse): T649‑p, TTK (rat): T654‑p, TTK (frog): T697‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  cell cycle regulation

T686-p - TTK (human)
Modsite: VkDSQVGtVNYMPPE SwissProt Entrez-Gene
Orthologous residues
TTK (human): T686‑p, TTK iso2 (human): T685‑p, TTK (mouse): T685‑p, TTK iso2 (mouse): T659‑p, TTK (rat): T664‑p, TTK (frog): T707‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S821-p - TTK (human)
Modsite: GQLVGLNsPNsILkA SwissProt Entrez-Gene
Orthologous residues
TTK (human): S821‑p, TTK iso2 (human): S820‑p, TTK (mouse): S820‑p, TTK iso2 (mouse): S794‑p, TTK (rat): S799‑p, TTK (frog): S844‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)