Curated Information
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Home > Curated Information Page > PubMed Id: 23734232
Mueller W, et al. (2013) Hierarchical organization of multi-site phosphorylation at the CXCR4 C terminus. PLoS One 8, e64975 23734232
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S331-p - CXCR4 (mouse)
Modsite: LNsMsRGssLkILSK SwissProt Entrez-Gene
Orthologous residues
CXCR4 (human): S324‑p, CXCR4 iso2 (human): S328‑p, CXCR4 (mouse): S331‑p, CXCR4 (rat): S321‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
EGF no change compared to control
NKH_477 no change compared to control
staurosporine phorbol_ester inhibit treatment-induced increase
CXCR4 (human) decrease E343K
CXCL12 increase
staurosporine CXCL12 inhibit treatment-induced increase

S332-p - CXCR4 (mouse)
Modsite: NsMsRGssLkILSKG SwissProt Entrez-Gene
Orthologous residues
CXCR4 (human): S325‑p, CXCR4 iso2 (human): S329‑p, CXCR4 (mouse): S332‑p, CXCR4 (rat): S322‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
EGF no change compared to control
NKH_477 no change compared to control
staurosporine phorbol_ester inhibit treatment-induced increase
CXCR4 (human) decrease E343K
CXCL12 increase
staurosporine CXCL12 inhibit treatment-induced increase

S345-p - CXCR4 (mouse)
Modsite: KGKRGGHssVsTESE SwissProt Entrez-Gene
Orthologous residues
CXCR4 (human): S338‑p, CXCR4 iso2 (human): S342‑p, CXCR4 (mouse): S345‑p, CXCR4 (rat): S335‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
EGF no change compared to control
NKH_477 no change compared to control
staurosporine phorbol_ester inhibit treatment-induced increase
CXCR4 (human) decrease E343K
CXCL12 increase
staurosporine CXCL12 no effect upon treatment-induced increase

S346-p - CXCR4 (mouse)
Modsite: GKRGGHssVsTESEs SwissProt Entrez-Gene
Orthologous residues
CXCR4 (human): S339‑p, CXCR4 iso2 (human): S343‑p, CXCR4 (mouse): S346‑p, CXCR4 (rat): S336‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
EGF no change compared to control
NKH_477 no change compared to control
staurosporine phorbol_ester inhibit treatment-induced increase
CXCR4 (human) decrease E343K
CXCL12 increase
staurosporine CXCL12 no effect upon treatment-induced increase

S348-p - CXCR4 (mouse)
Modsite: RGGHssVsTESEsss SwissProt Entrez-Gene
Orthologous residues
CXCR4 (human): S341‑p, CXCR4 iso2 (human): S345‑p, CXCR4 (mouse): S348‑p, CXCR4 (rat): S338‑p

S353-p - CXCR4 (mouse)
Modsite: sVsTESEsssFHSs_ SwissProt Entrez-Gene
Orthologous residues
CXCR4 (human): S346‑p, CXCR4 iso2 (human): S350‑p, CXCR4 (mouse): S353‑p, CXCR4 (rat): S343‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GRK2 (human) siRNA inhibition of enzyme
KINASE GRK3 (human) siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
EGF no change compared to control
NKH_477 no change compared to control
staurosporine phorbol_ester inhibit treatment-induced increase
CXCR4 (human) decrease E343K
CXCL12 increase
staurosporine CXCL12 no effect upon treatment-induced increase
siRNA CXCL12 inhibit treatment-induced increase siRNA for GRK2 and GRK3 decreses phosphorylation
Downstream Regulation
Effect of modification (function):  phosphorylation, receptor desensitization, inhibited, receptor internalization, induced

S354-p - CXCR4 (mouse)
Modsite: VsTESEsssFHSs__ SwissProt Entrez-Gene
Orthologous residues
CXCR4 (human): S347‑p, CXCR4 iso2 (human): S351‑p, CXCR4 (mouse): S354‑p, CXCR4 (rat): S344‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GRK3 (human) siRNA inhibition of enzyme
KINASE GRK2 (human) siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
EGF no change compared to control
NKH_477 no change compared to control
staurosporine phorbol_ester inhibit treatment-induced increase
CXCR4 (human) decrease E343K
CXCL12 increase
staurosporine CXCL12 no effect upon treatment-induced increase
siRNA CXCL12 inhibit treatment-induced increase siRNA for GRK2 and GRK3 decreses phosphorylation
Downstream Regulation
Effect of modification (function):  phosphorylation, receptor desensitization, inhibited, receptor internalization, induced

S359-p - CXCR4 (mouse)
Modsite: EsssFHSs_______ SwissProt Entrez-Gene
Orthologous residues
CXCR4 (human): S352‑p, CXCR4 iso2 (human): S356‑p, CXCR4 (mouse): S359‑p, CXCR4 (rat): S349‑p