Wang S, et al. (2013) Progesterone receptor A stability is mediated by glycogen synthase kinase-3β in the Brca1-deficient mammary gland. J Biol Chem 288, 26265-74 23880761

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

S26-p - PR iso2 (human) ▲
Modsite: KVLPRGLsPARQLLL SwissProt Entrez-Gene Orthologous residues PR (human): S190‑p, PR iso2 (human): S26‑p, PR (mouse): S191‑p, PR (rat): S190‑p, PR (chicken): ‑ Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HEK293T (epithelial), SF9 Cellular systems studied:  cell lines Species studied:  human, insect

S63-p - PR iso2 (human) ▲
Modsite: EVEEEDGsESEESAG SwissProt Entrez-Gene Orthologous residues PR (human): S227‑p, PR iso2 (human): S63‑p, PR (mouse): L228‑p, PR (rat): L227‑p, PR (chicken): P172‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HEK293T (epithelial), SF9 Cellular systems studied:  cell lines Species studied:  human, insect

S112-p - PR iso2 (human) ▲
Modsite: PKEDSRFsAPRVALV SwissProt Entrez-Gene Orthologous residues PR (human): S276‑p, PR iso2 (human): S112‑p, PR (mouse): S277‑p, PR (rat): S276‑p, PR (chicken): ‑ Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HEK293T (epithelial), SF9 Cellular systems studied:  cell lines Species studied:  human, insect

S130-p - PR iso2 (human) ▲
Modsite: APMAPGRsPLATTVM SwissProt Entrez-Gene Orthologous residues PR (human): S294‑p, PR iso2 (human): S130‑p, PR (mouse): S294‑p, PR (rat): S293‑p, PR (chicken): G219‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HEK293T (epithelial), SF9 Cellular systems studied:  cell lines Species studied:  human, insect

S164-p - PR iso2 (human) ▲

Modsite: RQLLEDEsYDGGAGA SwissProt Entrez-Gene Orthologous residues PR (human): S328‑p, PR iso2 (human): S164‑p, PR (mouse): S328‑p, PR (rat): S327‑p, PR (chicken): A247‑p Characterization Methods used to characterize site in vivo:  mass spectrometry (in vitro) Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human)

S236-p - PR iso2 (human) ▲
Modsite: GAEASARsPRSYLVA SwissProt Entrez-Gene Orthologous residues PR (human): S400‑p, PR iso2 (human): S236‑p, PR (mouse): S400‑p, PR (rat): S399‑p, PR (chicken): ‑ Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HEK293T (epithelial), SF9 Cellular systems studied:  cell lines Species studied:  human, insect

S390-p - PR iso2 (human) ▲

Modsite: PDSEASQsPQYsFES SwissProt Entrez-Gene Orthologous residues PR (human): S554‑p, PR iso2 (human): S390‑p, PR (mouse): S547‑p, PR (rat): S544‑p, PR (chicken): S408‑p Characterization Methods used to characterize site in vivo:  mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical), mammary gland, MCF10A1 (epithelial) Cellular systems studied:  cell lines, tissue Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes BIO decrease progestin_R5020 increase siRNA decrease siRNA for GSK3B decreases phosphorylation Downstream Regulation Effect of modification (function):  protein degradation, ubiquitination Effect of modification (process):  apoptosis, induced, carcinogenesis, altered, cell growth, inhibited

S394-p - PR iso2 (human) ▲
Modsite: ASQsPQYsFESLPQK SwissProt Entrez-Gene Orthologous residues PR (human): S558‑p, PR iso2 (human): S394‑p, PR (mouse): G551‑p, PR (rat): G548‑p, PR (chicken): S412‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HEK293T (epithelial), SF9 Cellular systems studied:  cell lines Species studied:  human, insect

S512-p - PR iso2 (human) ▲
Modsite: LSQRFTFsPGQDIQL SwissProt Entrez-Gene Orthologous residues PR (human): S676‑p, PR iso2 (human): S512‑p, PR (mouse): S669‑p, PR (rat): S666‑p, PR (chicken): S529‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HEK293T (epithelial), SF9 Cellular systems studied:  cell lines Species studied:  human, insect