Curated Information
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Home > Curated Information Page > PubMed Id: 23708000
Calvo F, et al. (2013) Mechanotransduction and YAP-dependent matrix remodelling is required for the generation and maintenance of cancer-associated fibroblasts. Nat Cell Biol 15, 637-46 23708000
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S908-p - LATS1 (mouse)
Modsite: HQRCLAHsLVGTPNY SwissProt Entrez-Gene
Orthologous residues
LATS1 (human): S909‑p, LATS1 (mouse): S908‑p, LATS1 (rat): S909‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MMTV-PyMT (breast cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T1078-p - LATS1 (mouse)
Modsite: EHAFYEFtFRRFFDD SwissProt Entrez-Gene
Orthologous residues
LATS1 (human): T1079‑p, LATS1 (mouse): T1078‑p, LATS1 (rat): T1079‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MMTV-PyMT (breast cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T183-p - MST1 (mouse)
Modsite: DtMAKRNtVIGTPFW SwissProt Entrez-Gene
Orthologous residues
MST1 (human): T183‑p, MST1 iso2 (human): T183‑p, MST1 (mouse): T183‑p, MST1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MMTV-PyMT (breast cell)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
blebbistatin no change compared to control
Y27632 no change compared to control
dasatinib no change compared to control

T180-p - MST2 (mouse)
Modsite: DtMAKRNtVIGTPFW SwissProt Entrez-Gene
Orthologous residues
MST2 (human): T180‑p, MST2 (mouse): T180‑p, MST2 (rat): T180‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MMTV-PyMT (breast cell)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
blebbistatin no change compared to control
Y27632 no change compared to control
dasatinib no change compared to control

S130-p - YAP1 (mouse)
Modsite: sPGtLTAsGVVsGPA SwissProt Entrez-Gene
Orthologous residues
YAP1 (human): T145‑p, YAP1 iso2 (human): T145‑p, YAP1 iso3 (human): T145‑p, YAP1 (mouse): S130‑p, YAP1 (rat): S127‑p, YAP1 iso3 (rat): S127‑p, YAP1 (sheep): T60‑p, YAP1 (cow): T73‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MMTV-PyMT (breast cell)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
blebbistatin no change compared to control
Y27632 no change compared to control
dasatinib no change compared to control

Y376-p - YAP1 (mouse)
Modsite: PFLNSGTyHSRDEst SwissProt Entrez-Gene
Orthologous residues
YAP1 (human): Y391‑p, YAP1 iso2 (human): Y375‑p, YAP1 iso3 (human): Y341‑p, YAP1 iso5 (human): Y337‑p, YAP1 iso6 (human): Y353‑p, YAP1 iso7 (human): Y357‑p, YAP1 iso8 (human): Y379‑p, YAP1 iso9 (human): Y395‑p, YAP1 (mouse): Y376‑p, YAP1 (rat): Y357‑p, YAP1 iso3 (rat): , YAP1 (sheep): Y290‑p, YAP1 (cow): Y319‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MMTV-PyMT (breast cell)
Cellular systems studied:  cell lines
Species studied:  mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TEAD1 (mouse) Induces transcription, induced co-immunoprecipitation
TEAD4 (mouse) Induces transcription, induced co-immunoprecipitation