Mallampalli RK, et al. (2013) Fbxl12 triggers G1 arrest by mediating degradation of calmodulin kinase I. Cell Signal 25, 2047-59 23707388

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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K59-ub - CAMK1A (human) ▲

Modsite: AKEALEGkEGSMENE SwissProt Entrez-Gene Orthologous residues CAMK1A (human): K59‑ub, CAMK1A (mouse): K59‑ub, CAMK1A (rat): K59‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  MLE (epithelial) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE FBXL12 (human) siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA FBXL12 (human) decrease E3-ubiquitin ligase FBXL12 siRNA decreases Downstream Regulation Effect of modification (function):  protein degradation

S10-p - p27Kip1 (human) ▲

Modsite: NVRVSNGsPsLErMD SwissProt Entrez-Gene Orthologous residues p27Kip1 (human): S10‑p, p27Kip1 (mouse): S10‑p, p27Kip1 (rat): S10‑p Characterization Methods used to characterize site in vivo:  immunoassay, immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma Relevant cell lines - cell types - tissues:  A549 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes FBXL12 (human) decrease serum increase KN-93 decrease

T157-p - p27Kip1 (human) ▲

Modsite: GIRkrPAtDDSSTQN SwissProt Entrez-Gene Orthologous residues p27Kip1 (human): T157‑p, p27Kip1 (mouse): A157‑p, p27Kip1 (rat): A157‑p Characterization Methods used to characterize site in vivo:  immunoassay, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma Relevant cell lines - cell types - tissues:  A549 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE CAMK1A (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CAMK1A (human) co-immunoprecipitation, phospho-antibody, activation of upstream enzyme, microscopy-colocalization, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, transfection of wild-type enzyme, modification site within consensus motif KINASE Akt1 (human) co-immunoprecipitation, phospho-antibody, activation of upstream enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes FBXL12 (human) decrease serum increase KN-93 decrease LY294002 decrease Downstream Regulation Effect of modification (function):  intracellular localization Effect of modification (process):  cell cycle regulation

T198-p - p27Kip1 (human) ▲

Modsite: PGLRRRQt_______ SwissProt Entrez-Gene Orthologous residues p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p Characterization Methods used to characterize site in vivo:  immunoassay, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma Relevant cell lines - cell types - tissues:  A549 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE CAMK1A (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Akt1 (human) co-immunoprecipitation, phospho-antibody, activation of upstream enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme KINASE CAMK1A (human) co-immunoprecipitation, phospho-antibody, activation of upstream enzyme, microscopy-colocalization, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, transfection of wild-type enzyme, modification site within consensus motif Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes FBXL12 (human) decrease serum increase KN-93 decrease LY294002 decrease Downstream Regulation Effect of modification (function):  intracellular localization Effect of modification (process):  cell cycle regulation

T170-p - p27Kip1 (mouse) ▲

Modsite: QNkRANRtEENVsDG SwissProt Entrez-Gene Orthologous residues p27Kip1 (human): T170‑p, p27Kip1 (mouse): T170‑p, p27Kip1 (rat): T170‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  MLE (epithelial) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CAMK1A (human) co-immunoprecipitation, phospho-antibody, activation of upstream enzyme, microscopy-colocalization, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, transfection of wild-type enzyme, modification site within consensus motif

T197-p - p27Kip1 (mouse) ▲

Modsite: KPGLRRQt_______ SwissProt Entrez-Gene Orthologous residues p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  MLE (epithelial) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CAMK1A (human) co-immunoprecipitation, phospho-antibody, activation of upstream enzyme, microscopy-colocalization, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, transfection of wild-type enzyme, modification site within consensus motif