Krishnamoorthy V, Donofrio AJ, Martin JL (2013) O-GlcNAcylation of αB-crystallin regulates its stress-induced translocation and cytoprotection. Mol Cell Biochem 379, 59-68 23543138

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S45-p - CRYAB (rat) ▲

Modsite: FSTATSLsPFYLRPP SwissProt Entrez-Gene Orthologous residues CRYAB (human): S45‑p, CRYAB (mouse): S45‑p, CRYAB (rat): S45‑p, CRYAB (rabbit): S45‑p, CRYAB (pig): S45‑p, CRYAB (cow): S45‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cells Species studied:  rat

S59-p - CRYAB (rat) ▲

Modsite: PSFLRAPsWIDTGLS SwissProt Entrez-Gene Orthologous residues CRYAB (human): S59‑p, CRYAB (mouse): S59‑p, CRYAB (rat): S59‑p, CRYAB (rabbit): S59‑p, CRYAB (pig): S59‑p, CRYAB (cow): S59‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cells Species studied:  rat

T170-gl - CRYAB (rat) ▲

Modsite: REEkPAVtAAPKK__ SwissProt Entrez-Gene Orthologous residues CRYAB (human): T170‑gl, CRYAB (mouse): A170‑gl, CRYAB (rat): T170‑gl, CRYAB (rabbit): T170‑gl, CRYAB (pig): T170‑gl, CRYAB (cow): T170‑gl Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  COS (fibroblast), myocyte-heart Cellular systems studied:  primary cells Species studied:  green monkey, mouse, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes heat_shock increase Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation, phosphorylation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays HSP27 (human) Induces co-immunoprecipitation Comments:  inhibits phosphorylation at S45 and S59