Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 23460737
Paster W, et al. (2013) GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. J Immunol 190, 3749-56 23460737
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

Y171-p - LAT iso2 (human)
Modsite: SMESIDDyVNVPESG SwissProt Entrez-Gene
Orthologous residues
LAT (human): Y200‑p, LAT iso2 (human): Y171‑p, LAT iso3 (human): Y207‑p, LAT iso4 (human): Y170‑p, LAT (mouse): Y175‑p, LAT (rat): Y175‑p
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
THEMIS (human) Induces pull-down assay
GRB2 (human) Induces pull-down assay

Y191-p - LAT iso2 (human)
Modsite: SLDGSREyVNVSQEL SwissProt Entrez-Gene
Orthologous residues
LAT (human): Y220‑p, LAT iso2 (human): Y191‑p, LAT iso3 (human): Y227‑p, LAT iso4 (human): Y190‑p, LAT (mouse): Y195‑p, LAT (rat): Y195‑p
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GRB2 (human) Induces pull-down assay
THEMIS (human) Induces pull-down assay

Y226-p - LAT iso2 (human)
Modsite: EEEGAPDyENLQELN SwissProt Entrez-Gene
Orthologous residues
LAT (human): Y255‑p, LAT iso2 (human): Y226‑p, LAT iso3 (human): Y262‑p, LAT iso4 (human): Y225‑p, LAT (mouse): Y235‑p, LAT (rat): Y234‑p
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GRB2 (human) Induces pull-down assay
THEMIS (human) Induces pull-down assay

Y95-p - THEMIS (human)
Modsite: IVADkTPyLTMEEIT SwissProt Entrez-Gene
Orthologous residues
THEMIS (human): Y95‑p, THEMIS iso4 (human): Y95‑p, THEMIS (mouse): Y96‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  293 (epithelial), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Lck (human)
KINASE ZAP70 (human)

Y174-p - THEMIS (human)
Modsite: LsQEGEFyECEDERI SwissProt Entrez-Gene
Orthologous residues
THEMIS (human): Y174‑p, THEMIS iso4 (human): Y174‑p, THEMIS (mouse): Y175‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  293 (epithelial), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ZAP70 (human)
KINASE Lck (human)

Y353-p - THEMIS (human)
Modsite: PREFPTAyDLEIAkS SwissProt Entrez-Gene
Orthologous residues
THEMIS (human): Y353‑p, THEMIS iso4 (human): Y353‑p, THEMIS (mouse): Y354‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  293 (epithelial), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ZAP70 (human)
KINASE Lck (human)

Y429-p - THEMIS (human)
Modsite: EAALLPLyMEGGFVE SwissProt Entrez-Gene
Orthologous residues
THEMIS (human): Y429‑p, THEMIS iso4 (human): Y429‑p, THEMIS (mouse): Y429‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  293 (epithelial), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Lck (human)
KINASE ZAP70 (human)

Y540-p - THEMIS (human)
Modsite: EEITEEQyyMMRRYE SwissProt Entrez-Gene
Orthologous residues
THEMIS (human): Y540‑p, THEMIS iso4 (human): Y540‑p, THEMIS (mouse): Y542‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  293 (epithelial), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Lck (human)
KINASE ZAP70 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ZAP70 (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
KINASE Lck (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP2 decrease

Y541-p - THEMIS (human)
Modsite: EITEEQyyMMRRYES SwissProt Entrez-Gene
Orthologous residues
THEMIS (human): Y541‑p, THEMIS iso4 (human): Y541‑p, THEMIS (mouse): Y543‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  293 (epithelial), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ZAP70 (human)
KINASE Lck (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Lck (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
KINASE ZAP70 (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP2 decrease

Y542-p - THEMIS (mouse)
Modsite: EEITEEQyyMMRRYE SwissProt Entrez-Gene
Orthologous residues
THEMIS (human): Y540‑p, THEMIS iso4 (human): Y540‑p, THEMIS (mouse): Y542‑p

Y543-p - THEMIS (mouse)
Modsite: EITEEQyyMMRRYES SwissProt Entrez-Gene
Orthologous residues
THEMIS (human): Y541‑p, THEMIS iso4 (human): Y541‑p, THEMIS (mouse): Y543‑p