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Home > Curated Information Page > PubMed Id: 22267324
Hengeveld RC, et al. (2012) Development of a chemical genetic approach for human aurora B kinase identifies novel substrates of the chromosomal passenger complex. Mol Cell Proteomics 11, 47-59 22267324
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T158-p - AHNAK (human)
Modsite: ItVtRRVtAytVDVT SwissProt Entrez-Gene
Orthologous residues
AHNAK (human): T158‑p, AHNAK iso2 (human): , AHNAK (mouse): T159‑p, AHNAK (rat): T159‑p, AHNAK iso2 (rat): T159‑p, AHNAK (cow): T159‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S7-p - AurB (human)
Modsite: _MAQkENsyPWPyGR SwissProt Entrez-Gene
Orthologous residues
AurB (human): S7‑p, AurB iso2 (human): S7‑p, AurB iso5 (human): S7‑p, AurB (mouse): A7‑p, AurB (rat): V7‑p, AurB (pig): T7‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T16-p - AurB (human)
Modsite: PWPyGRQtAPsGLST SwissProt Entrez-Gene
Orthologous residues
AurB (human): T16‑p, AurB iso2 (human): T16‑p, AurB iso5 (human): T16‑p, AurB (mouse): T16‑p, AurB (rat): T16‑p, AurB (pig): T16‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S255-p - CCDC86 (human)
Modsite: DRsKKRFsQMLQDkP SwissProt Entrez-Gene
Orthologous residues
CCDC86 (human): S255‑p, CCDC86 (mouse): S321‑p, CCDC86 (rat): S236‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S83-p - CDCA5 (human)
Modsite: PRRsPRIsFFLEKEN SwissProt Entrez-Gene
Orthologous residues
CDCA5 (human): S83‑p, CDCA5 (mouse): S82‑p, CDCA5 (rat): S45‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T39-p - CKAP2 (human)
Modsite: EHLLRRktLFAYKQE SwissProt Entrez-Gene
Orthologous residues
CKAP2 (human): T39‑p, CKAP2 iso4 (human): T39‑p, CKAP2 iso5 (human): T39‑p, CKAP2 (mouse): T52‑p, CKAP2 (rat): T52‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S876-p - COBLL1 (human)
Modsite: LQMQKRVsGHyVTSA SwissProt Entrez-Gene
Orthologous residues
COBLL1 (human): S876‑p, COBLL1 iso2 (human): S914‑p, COBLL1 (mouse): S958‑p, COBLL1 iso2 (mouse): S926‑p, COBLL1 (rat): S897‑p, COBLL1 (cow):
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T312-p - CSIG (human)
Modsite: KRQQARktAsVLskD SwissProt Entrez-Gene
Orthologous residues
CSIG (human): T312‑p, CSIG (mouse): A309‑p, CSIG (rat): A239‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S33-p - DDA1 (human)
Modsite: kAsNRRPsVyLPTRE SwissProt Entrez-Gene
Orthologous residues
DDA1 (human): S33‑p, DDA1 (mouse): S33‑p, DDA1 (rat): S33‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S594-p - DDX3 (human)
Modsite: rSKssrFsGGFGArD SwissProt Entrez-Gene
Orthologous residues
DDX3 (human): S594‑p, DDX3 iso2 (human): S578‑p, DDX3 (mouse): S594‑p, DDX3 (rat): S594‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S592-p - DDX3Y (human)
Modsite: RSKSNrFsGGFGArD SwissProt Entrez-Gene
Orthologous residues
DDX3Y (human): S592‑p, DDX3Y iso2 (human): S589‑p, DDX3Y (mouse): S592‑p, DDX3Y (rat): S586‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S22-p - DDX52 (human)
Modsite: kFDTRRFsADAArFQ SwissProt Entrez-Gene
Orthologous residues
DDX52 (human): S22‑p, DDX52 (mouse): S22‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T67-p - DEK (human)
Modsite: KKKVERLtMQVssLQ SwissProt Entrez-Gene
Orthologous residues
DEK (human): T67‑p, DEK iso2 (human): , DEK (mouse): T71‑p, DEK (rat): T69‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S511-p - DNAPK (human)
Modsite: EsEDHRAsGEVRtGk SwissProt Entrez-Gene
Orthologous residues
DNAPK (human): S511‑p, DNAPK iso2 (human): S511‑p, DNAPK (mouse): S511‑p, DNAPK (rat): S511‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S174-p - GBF1 (human)
Modsite: ICFEMRLsELLRKSA SwissProt Entrez-Gene
Orthologous residues
GBF1 (human): S174‑p, GBF1 iso2 (human): S174‑p, GBF1 (mouse): S174‑p, GBF1 (rat): S174‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

Y83-p - H2B (human)
Modsite: EAsrLAHyNkrstIt SwissProt Entrez-Gene
Orthologous residues
H2B (human): Y83‑p, H2B (mouse): Y83‑p, H2B (rat): Y83‑p, H2B (fruit fly): Y80‑p, H2B (cow): Y83‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

Y84-p - H2B1C (human)
Modsite: EAsrLAHyNkrstIt SwissProt Entrez-Gene
Orthologous residues
H2B1C (human): Y84‑p, H2B1C (mouse): Y84‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

Y84-p - H2B1D (human)
Modsite: EAsrLAHyNkrstIt SwissProt Entrez-Gene
Orthologous residues
H2B1D (human): Y84‑p, H2B1D (mouse): Y84‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

Y84-p - H2B1H (human)
Modsite: EAsrLAHyNkrstIt SwissProt Entrez-Gene
Orthologous residues
H2B1H (human): Y84‑p, H2B1H (mouse): Y84‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

Y84-p - H2B1K (human)
Modsite: EAsrLAHyNkrstIt SwissProt Entrez-Gene
Orthologous residues
H2B1K (human): Y84‑p, H2B1K (mouse): Y84‑p, H2B1K (rat): Y84‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

Y84-p - H2B1N (human)
Modsite: EAsrLAHyNkrstIt SwissProt Entrez-Gene
Orthologous residues
H2B1N (human): Y84‑p, H2B1N (mouse): Y84‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

Y84-p - H2B2E (human)
Modsite: EAsrLAHyNkrstIt SwissProt Entrez-Gene
Orthologous residues
H2B2E (human): Y84‑p, H2B2E (mouse): Y84‑p, H2B2E (rat): Y83‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S10-p - H3 (human)
Modsite: tkQtArkstGGkAPr SwissProt Entrez-Gene
Orthologous residues
H3 (human): S10‑p, H3 (mouse): S10‑p, H3 iso2 (mouse): S10‑p, H3 iso3 (mouse): S10‑p, H3 (rat): S10‑p, H3 iso3 (rat): S10‑p, H3 (pig): S10‑p, H3 (chicken): S10‑p, H3 (cow): S10‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AurB (human) transfection of wild-type enzyme, co-immunoprecipitation

S25-p - HMGN2 (human)
Modsite: kDEPQRRsARLsAkP SwissProt Entrez-Gene
Orthologous residues
HMGN2 (human): S25‑p, HMGN2 (mouse): S25‑p, HMGN2 (rat): S25‑p, HMGN2 (cow): S25‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AurB (human) transfection of wild-type enzyme, co-immunoprecipitation

S29-p - HMGN2 (human)
Modsite: QRRsARLsAkPAPPk SwissProt Entrez-Gene
Orthologous residues
HMGN2 (human): S29‑p, HMGN2 (mouse): S29‑p, HMGN2 (rat): S29‑p, HMGN2 (cow): S29‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AurB (human) transfection of wild-type enzyme, co-immunoprecipitation

S92-p - HP1 alpha (human)
Modsite: SESNKRksNFsNsAD SwissProt Entrez-Gene
Orthologous residues
HP1 alpha (human): S92‑p, HP1 alpha (mouse): S92‑p, HP1 alpha (rat): S92‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S255-p - HSP90B (human)
Modsite: PKIEDVGsDEEDDsG SwissProt Entrez-Gene
Orthologous residues
HSP90B (human): S255‑p, HSP90B (mouse): S255‑p, HSP90B (rat): S255‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S452-p - HSP90B (human)
Modsite: stNRRRLsELLRyHt SwissProt Entrez-Gene
Orthologous residues
HSP90B (human): S452‑p, HSP90B (mouse): S452‑p, HSP90B (rat): S452‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T59-p - INCENP (human)
Modsite: EPELMPKtPSQKNRR SwissProt Entrez-Gene
Orthologous residues
INCENP (human): T59‑p, INCENP iso2 (human): T59‑p, INCENP (mouse): T59‑p, INCENP (rat): T59‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S72-p - INCENP (human)
Modsite: RRKKRRIsyVQDENR SwissProt Entrez-Gene
Orthologous residues
INCENP (human): S72‑p, INCENP iso2 (human): S72‑p, INCENP (mouse): S72‑p, INCENP (rat): S72‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T494-p - INCENP (human)
Modsite: kVVRPLRtFLHtVQR SwissProt Entrez-Gene
Orthologous residues
INCENP (human): T494‑p, INCENP iso2 (human): T494‑p, INCENP (mouse): T468‑p, INCENP (rat): T481‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S518-p - INCENP (human)
Modsite: APRsVMKsFIKRNtP SwissProt Entrez-Gene
Orthologous residues
INCENP (human): S518‑p, INCENP iso2 (human): S518‑p, INCENP (mouse): S493‑p, INCENP (rat): S506‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S899-p - INCENP (human)
Modsite: tssAVWNsPPLQGAR SwissProt Entrez-Gene
Orthologous residues
INCENP (human): S899‑p, INCENP iso2 (human): S895‑p, INCENP (mouse): S867‑p, INCENP (rat): S883‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T6-p - K8 (human)
Modsite: __MsIRVtQksykVs SwissProt Entrez-Gene
Orthologous residues
K8 (human): T6‑p, K8 iso2 (human): T34‑p, K8 (mouse): T6‑p, K8 (rat): T6‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S374-p - KI-67 (human)
Modsite: ytTGRREsVNLGkSE SwissProt Entrez-Gene
Orthologous residues
KI‑67 (human): S374‑p, KI‑67 iso2 (human): , KI‑67 (mouse): S337‑p, KI‑67 (rat): S336‑p, KI‑67 iso4 (rat): S336‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S538-p - KI-67 (human)
Modsite: EAPTKRksLVMHtPP SwissProt Entrez-Gene
Orthologous residues
KI‑67 (human): S538‑p, KI‑67 iso2 (human): S178‑p, KI‑67 (mouse): S498‑p, KI‑67 (rat): S498‑p, KI‑67 iso4 (rat): S498‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S300-p - LMO7 (human)
Modsite: DMsyRRIsAVEPkTA SwissProt Entrez-Gene
Orthologous residues
LMO7 (human): S300‑p, LMO7 iso2 (human): S300‑p, LMO7 iso3 (human): S300‑p, LMO7 iso4 (human): S300‑p, LMO7 (mouse): S248‑p, LMO7 iso4 (mouse): S15‑p, LMO7 (rat): S248‑p, LMO7 iso3 (rat): S248‑p, LMO7 iso4 (rat): S15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S751-p - LMO7 (human)
Modsite: kWKDRRksYtsDLQK SwissProt Entrez-Gene
Orthologous residues
LMO7 (human): S751‑p, LMO7 iso2 (human): S751‑p, LMO7 iso3 (human): S417‑p, LMO7 iso4 (human): S751‑p, LMO7 (mouse): S699‑p, LMO7 iso4 (mouse): S132‑p, LMO7 (rat): S699‑p, LMO7 iso3 (rat): S365‑p, LMO7 iso4 (rat): S132‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T249-p - MgcRacGAP (human)
Modsite: WTRSRRktGtLQPWN SwissProt Entrez-Gene
Orthologous residues
MgcRacGAP (human): T249‑p, MgcRacGAP (mouse): S250‑p, MgcRacGAP (rat): S249‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S348-p - MISP (human)
Modsite: RRERGRPsLyVQRDI SwissProt Entrez-Gene
Orthologous residues
MISP (human): S348‑p, MISP (mouse): S318‑p, MISP (rat): S320‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T332-p - MPHOSPH10 (human)
Modsite: KESLKRVtFALPDDA SwissProt Entrez-Gene
Orthologous residues
MPHOSPH10 (human): T332‑p, MPHOSPH10 (mouse): T334‑p, MPHOSPH10 (rat): T329‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S1303-p - MYBBP1A (human)
Modsite: ARKKARLsLVIRsPs SwissProt Entrez-Gene
Orthologous residues
MYBBP1A (human): S1303‑p, MYBBP1A iso2 (human): S1303‑p, MYBBP1A (mouse): S1318‑p, MYBBP1A (rat): S1318‑p, MYBBP1A (cow): S1329‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S76-p - NAGK (human)
Modsite: RsLGLsLsGGDQEDA SwissProt Entrez-Gene
Orthologous residues
NAGK (human): S76‑p, NAGK iso2 (human): S122‑p, NAGK (mouse): S76‑p, NAGK (rat): S76‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S20-p - NSBP1 (human)
Modsite: RQEPKRRsARLsAML SwissProt Entrez-Gene
Orthologous residues
NSBP1 (human): S20‑p, NSBP1 (mouse): S18‑p, NSBP1 (rat): S18‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S24-p - NSBP1 (human)
Modsite: KRRsARLsAMLVPVt SwissProt Entrez-Gene
Orthologous residues
NSBP1 (human): S24‑p, NSBP1 (mouse): S22‑p, NSBP1 (rat): S22‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T1811-p - NuMA-1 (human)
Modsite: tRsARRRttQIINIt SwissProt Entrez-Gene
Orthologous residues
NuMA‑1 (human): T1811‑p, NuMA‑1 iso2 (human): T1797‑p, NuMA‑1 (mouse): T1793‑p, NuMA‑1 (rat): T1777‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S1969-p - NuMA-1 (human)
Modsite: QEtLRRAsMQPIQIA SwissProt Entrez-Gene
Orthologous residues
NuMA‑1 (human): S1969‑p, NuMA‑1 iso2 (human): S1955‑p, NuMA‑1 (mouse): S1951‑p, NuMA‑1 (rat): S1935‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S2047-p - NuMA-1 (human)
Modsite: kQADRRQsMAFsILN SwissProt Entrez-Gene
Orthologous residues
NuMA‑1 (human): S2047‑p, NuMA‑1 iso2 (human): S2033‑p, NuMA‑1 (mouse): S2029‑p, NuMA‑1 (rat): S2013‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T2055-p - NuMA-1 (human)
Modsite: MAFsILNtPkkLGNs SwissProt Entrez-Gene
Orthologous residues
NuMA‑1 (human): T2055‑p, NuMA‑1 iso2 (human): T2041‑p, NuMA‑1 (mouse): T2037‑p, NuMA‑1 (rat): T2021‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S240-p - NUSAP1 (human)
Modsite: VPPRGRLsVAstPIs SwissProt Entrez-Gene
Orthologous residues
NUSAP1 (human): S240‑p, NUSAP1 iso2 (human): S239‑p, NUSAP1 iso4 (human): S224‑p, NUSAP1 (mouse): S246‑p, NUSAP1 (rat): S234‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T2886-p - Plectin-1 (human)
Modsite: PVRNRRLtVNEAVKE SwissProt Entrez-Gene
Orthologous residues
Plectin‑1 (human): T2886‑p, Plectin‑1 iso2 (human): T2776‑p, Plectin‑1 iso3 (human): T2772‑p, Plectin‑1 iso4 (human): T2749‑p, Plectin‑1 iso5 (human): T2749‑p, Plectin‑1 iso6 (human): T2753‑p, Plectin‑1 iso8 (human): T2727‑p, Plectin‑1 iso9 (human): T2735‑p, Plectin‑1 (mouse): T2893‑p, Plectin‑1 iso3 (mouse): T2745‑p, Plectin‑1 iso6 (mouse): T2774‑p, Plectin‑1 (rat): T2889‑p, Plectin‑1 iso2 (rat): T2746‑p, Plectin‑1 (cow): T2846‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S110-p - PPHLN1 (human)
Modsite: RDGFRRksFyssHyA SwissProt Entrez-Gene
Orthologous residues
PPHLN1 (human): S110‑p, PPHLN1 iso2 (human): S117‑p, PPHLN1 iso3 (human): S55‑p, PPHLN1 iso5 (human): S62‑p, PPHLN1 (mouse): S124‑p, PPHLN1 (rat): S153‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S1013-p - PRRC2C (human)
Modsite: NDRPVRRsGPIKKPV SwissProt Entrez-Gene
Orthologous residues
PRRC2C (human): S1013‑p, PRRC2C iso2 (human): S770‑p, PRRC2C iso3 (human): S1013‑p, PRRC2C iso4 (human): S1013‑p, PRRC2C iso7 (human): S1015‑p, PRRC2C (mouse): S988‑p, PRRC2C iso5 (mouse): S988‑p, PRRC2C (rat): S1006‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S300-p - PTRF (human)
Modsite: SRDKLRksFtPDHVV SwissProt Entrez-Gene
Orthologous residues
PTRF (human): S300‑p, PTRF iso2 (human): S210‑p, PTRF (mouse): S302‑p, PTRF iso3 (mouse): , PTRF (rat): S302‑p, PTRF (cow): S300‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S618-p - RBM14 (human)
Modsite: LsDyrRLsEsQLsFR SwissProt Entrez-Gene
Orthologous residues
RBM14 (human): S618‑p, RBM14 (mouse): S618‑p, RBM14 (rat): S618‑p, RBM14 (cow): S618‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S649-p - RBM14 (human)
Modsite: HsDyArysGsyNDyL SwissProt Entrez-Gene
Orthologous residues
RBM14 (human): S649‑p, RBM14 (mouse): S649‑p, RBM14 (rat): S649‑p, RBM14 (cow): S649‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S147-p - RBM3 (human)
Modsite: QGGyDrysGGNyRDN SwissProt Entrez-Gene
Orthologous residues
RBM3 (human): S147‑p, RBM3 (mouse): S143‑p, RBM3 iso2 (mouse): S144‑p, RBM3 (rat): S146‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S326-p - RBMX (human)
Modsite: GyGGsRDsysssRsD SwissProt Entrez-Gene
Orthologous residues
RBMX (human): S326‑p, RBMX iso2 (human): S313‑p, RBMX iso3 (human): , RBMX (mouse): S326‑p, RBMX (rat): S326‑p, RBMX (cow): S326‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S326-p - RBMXL1 (human)
Modsite: GyGGsRDsysSSRsD SwissProt Entrez-Gene
Orthologous residues
RBMXL1 (human): S326‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S131-p - RDBP (human)
Modsite: SRRPQRksLyEsFVS SwissProt Entrez-Gene
Orthologous residues
RDBP (human): S131‑p, RDBP iso3 (human): S138‑p, RDBP (mouse): S131‑p, RDBP (rat): S131‑p, RDBP (cow): S131‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S2205-p - RIF1 (human)
Modsite: VNkVRRVsFADPIyQ SwissProt Entrez-Gene
Orthologous residues
RIF1 (human): S2205‑p, RIF1 iso2  (human): S2205‑p, RIF1 (mouse): S2153‑p, RIF1 (rat): S2021‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S104-p - RPL21 (human)
Modsite: kHSKsRDsFLkRVKE SwissProt Entrez-Gene
Orthologous residues
RPL21 (human): S104‑p, RPL21 (mouse): S104‑p, RPL21 (rat): S104‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S130-p - RPL8 (human)
Modsite: RGKLARAsGNyATVI SwissProt Entrez-Gene
Orthologous residues
RPL8 (human): S130‑p, RPL8 (mouse): S130‑p, RPL8 (rat): S130‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T118-p - RPS10 (human)
Modsite: GERPARLtrGEADRD SwissProt Entrez-Gene
Orthologous residues
RPS10 (human): T118‑p, RPS10 (mouse): T118‑p, RPS10 (rat): T118‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S462-p - SAS10 (human)
Modsite: RKEEQRYsGELsGIR SwissProt Entrez-Gene
Orthologous residues
SAS10 (human): S462‑p, SAS10 (mouse): S452‑p, SAS10 (rat): S453‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S261-p - SH2D4A (human)
Modsite: REDYKRLsLGAQKGR SwissProt Entrez-Gene
Orthologous residues
SH2D4A (human): S261‑p, SH2D4A (mouse): S232‑p, SH2D4A (rat): S233‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

T494-p - SPT16 (human)
Modsite: EEAkRRLtEQkGEQQ SwissProt Entrez-Gene
Orthologous residues
SPT16 (human): T494‑p, SPT16 (mouse): T494‑p, SPT16 (rat): T494‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)

S37-p - SSH3 (human)
Modsite: SRLQRRQsFAVLRGA SwissProt Entrez-Gene
Orthologous residues
SSH3 (human): S37‑p, SSH3 iso4 (human): S37‑p, SSH3 (mouse): S38‑p, SSH3 (rat): S37‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Comments:  HeLa mitotic cell lysate + recombinant AurB L154G/H250Y (gatekeeper/suppressor double mutant)