Chocarro-Calvo A, et al. (2013) Glucose-induced β-catenin acetylation enhances Wnt signaling in cancer. Mol Cell 49, 474-86 23273980

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K354-ac - CTNNB1 (human) ▲

Modsite: LSVCSsNkPAIVEAG SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): K354‑ac, CTNNB1 (mouse): K354‑ac, CTNNB1 (rat): K354‑ac, CTNNB1 (rabbit): K354‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma, ovarian cancer, pancreatic cancer, pancreatic carcinoma Relevant cell lines - cell types - tissues:  Aspc1 (pancreatic), HT-29 (intestinal), MDA-MB-231 (breast cell), OVCAR3 (ovarian), STC-1 (endocrine) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE p300 (human) co-immunoprecipitation, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme DEACETYLASE SIRT1 (human) co-immunoprecipitation, pharmacological activator of upstream enzyme, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes resveratrol decrease nicotinamide increase lithium, glucose increase C646 glucose, lithium inhibit treatment-induced increase resveratrol glucose, lithium inhibit treatment-induced increase nicotinamide glucose, lithium augment treatment-induced increase Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays LEF-1 (human) Induces co-immunoprecipitation, pull-down assay