Cho IH, et al. (2013) SPIN90 phosphorylation modulates spine structure and synaptic function. PLoS One 8, e54276 23342115

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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Y85-p - NCKIPSD (human) ▲

Modsite: AMRDGGkySLEQRGV SwissProt Entrez-Gene Orthologous residues NCKIPSD (human): Y85‑p, NCKIPSD iso3 (human): Y85‑p, NCKIPSD (mouse): Y85‑p, NCKIPSD (rat): Y85‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  COS (fibroblast), HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cells Species studied:  human Downstream Regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PSD-95 (human) Induces co-immunoprecipitation Comments:  regulate spine size and synaptic function

Y161-p - NCKIPSD (human) ▲

Modsite: LGADGGLyQIPLPSS SwissProt Entrez-Gene Orthologous residues NCKIPSD (human): Y161‑p, NCKIPSD iso3 (human): Y161‑p, NCKIPSD (mouse): Y161‑p, NCKIPSD (rat): Y161‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  COS (fibroblast), HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cells Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Src (human) Downstream Regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PSD-95 (human) Induces co-immunoprecipitation Comments:  regulate spine size and synaptic function

Y227-p - NCKIPSD (human) ▲

Modsite: STSLDTLyTSSSPSE SwissProt Entrez-Gene Orthologous residues NCKIPSD (human): Y227‑p, NCKIPSD iso3 (human): Y220‑p, NCKIPSD (mouse): Y220‑p, NCKIPSD (rat): Y220‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  COS (fibroblast), HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cells Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Src (human) Downstream Regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PSD-95 (human) Induces co-immunoprecipitation Comments:  regulate spine size and synaptic function

Y419-p - Src (human) ▲

Modsite: RLIEDNEytARQGAk SwissProt Entrez-Gene Orthologous residues Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y418‑p, Src iso1 (mouse): Y424‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  COS (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PP2 decrease