Curated Information
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Home > Curated Information Page > PubMed Id: 23332762
Yan J, et al. (2013) Inactivation of BAD by IKK inhibits TNFα-induced apoptosis independently of NF-κB activation. Cell 152, 304-15 23332762
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S26-p - BAD (mouse)
Modsite: KsDPGIRsLGSDAGG SwissProt Entrez-Gene
Orthologous residues
BAD (human): , BAD (mouse): S26‑p, BAD (rat): S26‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Enzymes shown to modify site in vitro
Type Enzyme
KINASE IKKB (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) siRNA inhibition of enzyme, genetic knockout/knockin of upstream enzyme, transfection of constitutively active enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA decrease IKKB siRNA
TNF increase
TNF NFkB-p65 (mouse) no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  activity, inhibited, intracellular localization, molecular association, regulation, phosphorylation
Effect of modification (process):  apoptosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (mouse) Induces co-immunoprecipitation
Bcl-xL (mouse) Disrupts co-immunoprecipitation
Comments:  induces phosphorylation at S112, S136 and S155

S112-p - BAD (mouse)
Modsite: ETRsRHssyPAGtEE SwissProt Entrez-Gene
Orthologous residues
BAD (human): S75‑p, BAD (mouse): S112‑p, BAD (rat): S113‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
TNF IKKB (mouse) augment treatment-induced increase IKKB knockout inhibits treatment
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  apoptosis, inhibited

S136-p - BAD (mouse)
Modsite: PFRGRsRsAPPNLWA SwissProt Entrez-Gene
Orthologous residues
BAD (human): S99‑p, BAD (mouse): S136‑p, BAD (rat): S137‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
TNF IKKB (mouse) augment treatment-induced increase IKKB knockout inhibits treatment
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  apoptosis, inhibited

S155-p - BAD (mouse)
Modsite: GRELRRMsDEFEGsF SwissProt Entrez-Gene
Orthologous residues
BAD (human): S118‑p, BAD (mouse): S155‑p, BAD (rat): S156‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
TNF IKKB (mouse) augment treatment-induced increase IKKB knockout inhibits treatment
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  apoptosis, inhibited

S177-p - IKKB (mouse)
Modsite: AKELDQGsLCTsFVG SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S177‑p, IKKB (mouse): S177‑p, IKKB (rat): S177‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  apoptosis, inhibited
Comments:  induces phosphorylation of BAD

S181-p - IKKB (mouse)
Modsite: DQGsLCTsFVGTLQy SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  apoptosis, inhibited
Comments:  induces phosphorylation of BAD