Curated Information
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Home > Curated Information Page > PubMed Id: 23325788
Hatakeyama H, Kanzaki M (2013) Regulatory mode shift of Tbc1d1 is required for acquisition of insulin-responsive GLUT4-trafficking activity. Mol Biol Cell 24, 809-17 23325788
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S80-p - ACC1 (human)
Modsite: LHIRssMsGLHLVkQ SwissProt Entrez-Gene
Orthologous residues
ACC1 (human): S80‑p, ACC1 iso2 (human): S22‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
acadesine increase

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
acadesine increase slight increase

S237-p - TBC1D1 (human)
Modsite: RPMRKsFsQPGLRSL SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S237‑p, TBC1D1 iso2 (human): S237‑p, TBC1D1 (mouse): S231‑p, TBC1D1 iso2 (mouse): S231‑p, TBC1D1 (rat): S231‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
acadesine increase
ionomycin increase

S565-p - TBC1D1 (human)
Modsite: SsFKLLGssEDLssD SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S565‑p, TBC1D1 iso2 (human): S565‑p, TBC1D1 (mouse): S559‑p, TBC1D1 iso2 (mouse): S559‑p, TBC1D1 (rat): S559‑p

S566-p - TBC1D1 (human)
Modsite: sFKLLGssEDLssDs SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): S566‑p, TBC1D1 iso2 (human): S566‑p, TBC1D1 (mouse): S560‑p, TBC1D1 iso2 (mouse): S560‑p, TBC1D1 (rat): S560‑p

T596-p - TBC1D1 (human)
Modsite: AFRRRANtLsHFPIE SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): T596‑p, TBC1D1 iso2 (human): T596‑p, TBC1D1 (mouse): T590‑p, TBC1D1 iso2 (mouse): T590‑p, TBC1D1 (rat): T590‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
acadesine no change compared to control
ionomycin no change compared to control