Schlottmann S, et al. (2012) Acetylation Increases EWS-FLI1 DNA Binding and Transcriptional Activity. Front Oncol 2, 107 22973553

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K240-ac - FLI1 (human) ▲

Modsite: NMNsGLNksPPLGGA SwissProt Entrez-Gene Orthologous residues FLI1 (human): K240‑ac, FLI1 (mouse): K240‑ac, FLI1 (rat): K240‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  bone cancer, Ewing's sarcoma Relevant cell lines - cell types - tissues:  COS7 (fibroblast), TC-32 Cellular systems studied:  cell lines Species studied:  green monkey, human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE PCAF (human) ACETYLTRANSFERASE CBP (human) ACETYLTRANSFERASE p300 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE PCAF (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme ACETYLTRANSFERASE p300 (human) co-immunoprecipitation, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes trichostatin_A increase Downstream Regulation Effect of modification (process):  transcription, induced

K252-ac - FLI1 (human) ▲

Modsite: GGAQTISkNTEQRPQ SwissProt Entrez-Gene Orthologous residues FLI1 (human): K252‑ac, FLI1 (mouse): K252‑ac, FLI1 (rat): K252‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  bone cancer, Ewing's sarcoma Relevant cell lines - cell types - tissues:  COS7 (fibroblast), TC-32 Cellular systems studied:  cell lines Species studied:  green monkey, human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE CBP (human) ACETYLTRANSFERASE p300 (human) ACETYLTRANSFERASE PCAF (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE PCAF (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme ACETYLTRANSFERASE p300 (human) co-immunoprecipitation, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes trichostatin_A increase Downstream Regulation Effect of modification (process):  transcription, induced

K380-ac - FLI1 (human) ▲

Modsite: PTESSMYkYPSDISY SwissProt Entrez-Gene Orthologous residues FLI1 (human): K380‑ac, FLI1 (mouse): K380‑ac, FLI1 (rat): K380‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  bone cancer, Ewing's sarcoma Relevant cell lines - cell types - tissues:  COS7 (fibroblast), TC-32 Cellular systems studied:  cell lines Species studied:  green monkey, human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE PCAF (human) ACETYLTRANSFERASE CBP (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE PCAF (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme ACETYLTRANSFERASE p300 (human) co-immunoprecipitation, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes trichostatin_A increase Downstream Regulation Effect of modification (process):  transcription, induced

K397-ac - FLI1 (human) ▲

Modsite: SYHAHQQkVNFVPPH SwissProt Entrez-Gene Orthologous residues FLI1 (human): K397‑ac, FLI1 (mouse): K397‑ac, FLI1 (rat): K397‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  bone cancer, Ewing's sarcoma Relevant cell lines - cell types - tissues:  COS7 (fibroblast), TC-32 Cellular systems studied:  cell lines Species studied:  green monkey, human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE PCAF (human) ACETYLTRANSFERASE CBP (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE PCAF (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme ACETYLTRANSFERASE p300 (human) co-immunoprecipitation, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes trichostatin_A increase Downstream Regulation Effect of modification (process):  transcription, induced